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Benchmark Scientific - E4500-LD - SmartGlow Loading Dye with Safe Green Stain, 6X concentration, 1.0mL

MFR SKU: E4500-LD

ITEM #: 11316083

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Lists At: $122.50 You Save: $18.37

$104.13

Stock Status: SHIPS SOON (reserve your unit today)
Average lead time for this brand: less than a week. Specific products may vary.

ITEM #: 11316083

Replaces hazardous ethidium bromide (Et. Br)
Better sensitivity than Et. Br (down to 0.1ng)
E x citation of both UV and blue light
Ships at ambient temperature (store at 4°C)
Two types: PS (Pre-Stain) or LD (Loading Dye)
Compatible with Smart. Blue Transilluminator

Smart. Glow Pre Stain can be used the same way as Ethidium Bromide for agarose and polyacrylamide gel electrophoresis. The stain emits green fluorescence when bound to...

ITEM #: 11316083

Replaces hazardous ethidium bromide (Et. Br)
Better sensitivity than Et. Br (down to 0.1ng)
E x citation of both UV and blue light
Ships at ambient temperature (store at 4°C)
Two types: PS (Pre-Stain) or LD (Loading Dye)
Compatible with Smart. Blue Transilluminator

Smart. Glow Pre Stain can be used the same way as Ethidium Bromide for agarose and polyacrylamide gel electrophoresis. The stain emits green fluorescence when bound to dsDNA or ssDNA and emits red fluorescence when bound to RNA. Smart. Glow stain has two e x citation peaks at appro x imately 290nm (UV light) and 490nm (Blue light).
Protocol:

Prepare 100ml of agarose or polyacrylamide solution, heat to boil.

Add 5ul of Smart. Glow stain to the gel solution.

Mi x gently to and make sure there are no air bubbles in the mi x ture

Allow the solution to cool to about 60°C to 70°C then pour the gel(s).

For enhanced results, you can optionally add 5ul of Smart. Glow stain per 100ml of the running buffer. Adding the stain to the running buffer can help in increasing sensitivity and detecting small quantities of nucleic acid.

After the separation is complete, view the gel using a UV or blue light illuminator.

Using Smart. Glow Loading Dye , it is not required to add any stain to the gel or running buffer. Smart. Glow Loading Dye is supplied at 6X concentration, and only needs to be added to the samples before pipetting into the gel wells. This method eliminates the possibility of contamination of glassware, plasticware, and the gel running tank as can happen with Et. Br. After electrophoretic separation, view and document your results using a UV or Blue Light Illuminator.
Protocol:

Prepare 100ml of agarose or polyacrylamide solution

Mi x gently to and make sure there are no air bubbles in the mi x ture

Allow the solution to cool to about 60°C to 70°C then pour the gel(s).

Mi x samples and DNA markers with Smart. Glow Loading Dye at a 1:5 (dye to sample) dilution.

After the separation is complete, view the gel using a UV or blue light illuminator.

Smart. Glow products are considered safer than Ethidium Bromide. They are non-carcinogenic as determined by the Ames-test, with negative results in both mouse marrow chromophilous erythrocyte micronucleus and mouse spermary spermatocycte chromosomal aberration tests.
Smart. Glow Loading Dye with Safe Green Stain, 6X concentration, 1.0mL
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Benchmark Scientific - E4500-LD - SmartGlow Loading Dye with Safe Green Stain, 6X concentration, 1.0mL

Item #: 11316083

MFR SKU: E4500-LD

Benchmark Scientific - E4500-LD - SmartGlow Loading Dye with Safe Green Stain, 6X concentration, 1.0mL

Benchmark Scientific - E4500-LD - SmartGlow Loading Dye with Safe Green Stain, 6X concentration, 1.0mL

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