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Bio Basic - DB0154.SIZE.25ML - DEPC, 25mL

  • MFR SKU: DB0154.SIZE.25ML
  • ITEM #: 12934059
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ITEM #: 12934059
DEPC: Molecular formula: C6H10O5 Formula weight: 162.1 Density: 1.12 g/mL Molarity: 6.9 M Refractive Inde x : 1.398 at 20oC Diethyl Pyrocarbonate (DEPC) is sensitive to moisture and to pH; it decomposes to ethanol and carbon dio x ide in aqueous solution. It decomposes at 155°C. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen. DEPC is effective as a nuclease inhibitor. (It reacts with many enzymes containing -NH, -SH or - OH groups in their active...
ITEM #: 12934059
DEPC: Molecular formula: C6H10O5 Formula weight: 162.1 Density: 1.12 g/mL Molarity: 6.9 M Refractive Inde x : 1.398 at 20oC Diethyl Pyrocarbonate (DEPC) is sensitive to moisture and to pH; it decomposes to ethanol and carbon dio x ide in aqueous solution. It decomposes at 155°C. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen. DEPC is effective as a nuclease inhibitor. (It reacts with many enzymes containing -NH, -SH or - OH groups in their active sites. Typically, a 0.1% solution is used to inactive RNase (one milliliter of DEPC is added to 1 liter of water). The DEPC will not immediately dissolve, as evidenced by the appearance of globules. The mi x ture should be stirred until the globules disappear. The DEPC hydrolyzes over time to form ethanol and carbon dio x ide. At this time the solution may be autoclaved to destroy the DEPC. DEPC has been used as a gentle esterifying agent and preservative. DEPC acts as a condensing agent between lysine e-amine groups and the carbo x yl groups of aspartic or glutamic acid. It modifies purine residues in RNA by carbo x yethylation of histidyl residues. It is considered sufficient to autoclave 0.1% DEPC solutions for 15 minutes per liter. A method to assay for DEPC uses 5-thio-2- nitrobenzoate. For general information and background, see references
DEPC, 25mL
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Bio Basic - DB0154.SIZE.25ML - DEPC, 25mL

  • Item #: 12934059
  • MFR SKU: DB0154.SIZE.25ML
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