Biomiga - QP1318-03 - One Step qRT-PCR 2x Mix 12.5 mL

ITEM #: 23533148
Over 5 million reactions shipped for COVID-19 RT-qPCR detection

• Easy-to-use two tube system: Enzyme Mi x (50X) and Reaction Mi x (2X)
• Broad range of RT reaction temperature (40-55oC)
• Broad dynamic range of detection
• Hot start PCR system
• Fully optimized RT-qPCR buffer and robustness
• High sensitivity, specificity, and reproducibility
• Ideal for probe based COVID-19 viral RNA detection and quantitation

The One-Step qRT-PCR System, No ROX or Low Ro x is consist of Enzyme Mi x and 2X Reaction Mi x , It is designed for the reverse transcription (RT) and polymerase chain reaction (PCR) amplification of a specific target RNA from either total RNA or mRNA in a single tube for the detection and quantification of RNA using real-time detection instruments. The proprietary Enzyme Mi x contains an engineered Script Reverse Transcriptase (RNaseH minus), Hot Start Taq DNA polymerase, stabilizers. 2X Reaction Mi x consists of a proprietary buffer system, 6 mM MgCl2, dNTPs, enhancers and stabilizers. It also contains an optimal concentration of ROX for instruments that require low ROX for a reference signal. Please consult our website and Instrument Compatibility section below for the correct product for your real-time PCR system.
Hot Start Taq DNA polymerase is an antibody-inactivated hot-start enzyme designed to block polymerase activity between ambient to RT reaction temperature. Script RT enzyme can synthesize cDNA at a temperature range of 40-55oC. Once the PCR step reaches denaturation temperature (95oC), Taq DNA polymerase activity is restored and the resulting PCR e x hibits higher sensitivity, specificity and yield.
The system enables highly sensitive detection from as few as 10 copies of SARS-CoV-2 target genes (N1 and N2), with a broad dynamic range that supports accurate quantification of mRNA copies. Sufficient reagents are provided for 125, 500, or 1, 250 amplification reactions of 20 μl each.
One Step qRT-PCR 2x Mix 12.5 mL
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