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Cytiva - 17075601 - Glutathione Sepharose 4B, 10 mLItem must be purchased in units of 1

  • MFR SKU: 17075601
  • ITEM #: 3026808
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ITEM #: 3026808
Glutathione Sepharose 4B is an affinity chromatography resin for batch purification of GST-tagged proteins and when high binding capacity is required.
  • High capacity, single-step glutathione S-transferase (GST) tagged protein purification.
  • Supplied as pre-swollen bulk (loose) resin.
  • Chemically stable with all commonly used aqueous buffers.
  • Suitable for batch GST tagged protein purification and scale-up.
  • Used in GST pull-down assays to study protein-protein interactions.
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ITEM #: 3026808
Glutathione Sepharose 4B is an affinity chromatography resin for batch purification of GST-tagged proteins and when high binding capacity is required.
  • High capacity, single-step glutathione S-transferase (GST) tagged protein purification.
  • Supplied as pre-swollen bulk (loose) resin.
  • Chemically stable with all commonly used aqueous buffers.
  • Suitable for batch GST tagged protein purification and scale-up.
  • Used in GST pull-down assays to study protein-protein interactions.
Successful use in published research
  • GST pull-down assay to study the interaction of an RNA binding protein with a core subunit of a deadenylase complex
  • Study a protein-protein interaction network that regulates primary ciliogenesis
  • Determine the role of importin 8 in nuclear import of a eukaryotic translation initiation factor
Glutathione Sepharose 4B can be packed into the following empty column types: C, Tricorn, XK, and Hi. Scale.

Compatible empty columnsConvenient prepacked columns

Gluthatione Sepharose 4B resin is also available in the following formats:
  • GSTrap 4B columns: Hi. Trap convenient format that can be used with a syringe, a peristaltic pump, or a chromatography system
  • GST Multi. Trap 4B: 96 well plate format for high throughput screening and small scale purification using a centrifuge or vacuum equipment
  • GST Gravi. Trap: Gravity flow columns for direct purification of both clarified and unclarified cell lysates
  • GST Spin. Trap: Microcentrifuge based spin columns for rapid small-scale purification of GST-tagged proteins
Other products for recombinant protein purification

Glutathione Sepharose 4B is optimized for high yield batch purification. offers other Life Sciences solutions for purifying GST fusion proteins. Use product selector to find GST-tagged protein purification resins and prepacked columns that meet your needs.
This listing is for package of 10 mL.
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Cytiva - 17075601 - Glutathione Sepharose 4B, 10 mLItem must be purchased in units of 1

  • Item #: 3026808
  • MFR SKU: 17075601
Technical Specs
  • Particle Size: 45 µm-165 µm
  • Ligand: Glutathione
  • Particle size, d 50V : ~90 ?m ( Median particle size of the cumulative volume distribution. )
  • Matrix: Agarose, 4%
  • Ligand Coupling Method: Epoxy activation
  • Binding Capacity/ml Chromatography Medium: > 25 mg horse liver GST/mL resin ( Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution. )
  • pH stability, operational: 4–13 ( pH range where resin can be operated without significant change in function. )
  • pH stability, CIP: 4–13 ( pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function. )
  • Storage: 4 to 30°C, 20% Ethanol
  • Chemical stability: Commonly used aqueous buffers
  • Licensing: For licensing information, see the Licensing Statements page in the About Us section.
  • Chromatography technique: GST-tagged protein purification
  • Pack size: 10 mL
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