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Cytiva - 17505301 - HiTrap Q FFItem must be purchased in units of 1

  • MFR SKU: 17505301
  • ITEM #: 3527814
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ITEM #: 3527814
Hi. Trap Q FF is a strong anion exchange chromatography column for small-scale protein purification.
  • Packed with Q Sepharose Fast Flow strong anion exchange resin.
  • Convenient and affordable for fast ion exchange separations, either alone or connected in series.
  • High flow rates and good scale-up potential.
  • Resin made up of 6% crosslinked agarose and 90 µm particle size.
  • pH stable: 2-12 operational, 2-14 for cleaning.
  • Convenient Hi. Trap format...
ITEM #: 3527814
Hi. Trap Q FF is a strong anion exchange chromatography column for small-scale protein purification.
  • Packed with Q Sepharose Fast Flow strong anion exchange resin.
  • Convenient and affordable for fast ion exchange separations, either alone or connected in series.
  • High flow rates and good scale-up potential.
  • Resin made up of 6% crosslinked agarose and 90 µm particle size.
  • pH stable: 2-12 operational, 2-14 for cleaning.
  • Convenient Hi. Trap format for easy connection to a syringe, peristaltic pump, or chromatography system such as an ÄKTA system.
Straightforward purification scale-up

Q Sepharose Fast Flow resin uses the Sepharose Fast Flow ion exchange platform, which has been used for many years in industry.
Composed of crosslinked 6% agarose beads with quaternary amine strong anion exchange groups, this resin has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. The resin’s 90 µm bead size yields rapid flow rates, making it simple to scale up the purification.
For even faster scale up with better binding capacity, choose our Capto Q resin.Successful use in published research

This resin is utilized in a variety of research applications, including:
  • Purifying RECQ5 helicase to study how it is recruited to sites of DNA damage
  • Purification of recombinant proteins to study a potential antimicrobial for Bacillus anthracis vegetative cells
  • Isolation of cPLA2 from human platelets to evaluate inhibitors
Other Q Sepharose Fast Flow formats

This strong anion exchanger is available in the following formats:
  • Bulk Q resin - Q Sepharose Fast Flow
  • Hi. Screen columns for method and process development
  • Hi. Prep columns for convenient scale-up.

This listing is for package of 5 × 1 mL.
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Cytiva - 17505301 - HiTrap Q FFItem must be purchased in units of 1

  • Item #: 3527814
  • MFR SKU: 17505301
Technical Specs
  • Bed Volume: 1 ml
  • Storage Conditions: 4 to 30°C, 20% Ethanol
  • Pressure Max. [Over the Packed Bed During Operation]: 5 bar [0.5 MPa] (70 psi)
  • Application: For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules
  • BioProcess resin: Yes
  • Ligand: Quaternary amine
  • Particle size, d50V: ~90 µm1)
  • Ion Exchanger Type: Strong anion exchanger
  • Matrix: cross-linked, 6% agarose particles
  • Ionic Capacity: 0.18-0.24 mmol Cl-/ml medium
  • PH stability, operational: 2-122)
  • PH stability, CIP: 2-143)
  • PH ligand fully charged: Entire pH range4)
  • Pressure/Flow Specification: 400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm
  • Exclusion Limit [Mr] [Globular Proteins]: ~4 × 106
  • Storage: 4 to 30°C, 20% Ethanol
  • Chemical Stability: Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol5)
  • Certificate of Analysis: Yes
  • Column i.d.: 7 mm
  • Bed Dimensions: 7 × 25 mm
  • Complete Packsize: 1 ml
  • Material [Column Hardware]: Polypropylene (PP)
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