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Cytiva - 17505401 - HiTrap SP FFItem must be purchased in units of 1

  • MFR SKU: 17505401
  • ITEM #: 3527815
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ITEM #: 3527815
HiTrap SP FF is prepacked with SP Sepharose Fast Flow, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.
Q, SP, DEAE, and CM Sepharose Fast FLow are based on a robust, 6% highly cross-linked beaded Agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast FLow (high sub) is based on 4% highly cross-linked beaded Agarose. This results in a medium with higher porosity, which is particularly...
ITEM #: 3527815
HiTrap SP FF is prepacked with SP Sepharose Fast Flow, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.
Q, SP, DEAE, and CM Sepharose Fast FLow are based on a robust, 6% highly cross-linked beaded Agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast FLow (high sub) is based on 4% highly cross-linked beaded Agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass Proteins.
• Convenient and affordable for fast, easy ion exchange separations either alone or connected in series. • The industry standard for ion exchange chromatography. • High fLow rates and good scale-up potential. • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient. • Predictable scale-up
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)
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HiTrap is a registered trademark of Cytiva
Sepharose is a registered trademark of Cytiva
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Cytiva - 17505401 - HiTrap SP FFItem must be purchased in units of 1

  • Item #: 3527815
  • MFR SKU: 17505401
Technical Specs
  • Bed Volume: 1 ml
  • Storage Conditions: 4 to 30°C, 0.2 M Sodium Acetate in 20% Ethanol
  • Pressure Max. [Over the Packed Bed During Operation]: 5 bar [0.5 MPa] (70 psi)
  • Application: For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules
  • BioProcess resin: Yes
  • Ligand: Sulphopropyl
  • Particle size, d50V: ~90 µm1)
  • Ion Exchanger Type: Strong cation exchanger
  • Matrix: cross-linked agarose, 6%, spherical
  • Ionic Capacity: 0.18-0.25 mmol H+/ml medium
  • PH stability, operational: 4-132)
  • PH stability, CIP: 3-143)
  • PH ligand fully charged: Entire pH range4)
  • Pressure/Flow Specification: 400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm
  • Exclusion Limit [Mr] [Globular Proteins]: ~4 × 106
  • Storage: 4 to 30°C, 20% Ethanol + 0.2 M Sodium Acetate
  • Chemical Stability: Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol5)
  • Bed Dimensions: 7 × 25 mm
  • Column i.d.: 7 mm
  • Complete Packsize: 1 ml
  • Material [Column Hardware]: Polypropylene (PP)
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