Image is an illustration, may not reflect the exact product sold.


Other products by Cytiva

Cytiva - 17507901 - HiTrap rProtein A FF, 5 x 1 mlItem must be purchased in units of 1

  • MFR SKU: 17507901
  • ITEM #: 3527818
    • 0 out of 5 stars
    • 5 Star
      0 %
    • 4 Star
      0 %
    • 3 Star
      0 %
    • 2 Star
      0 %
    • 1 Star
      0 %
  • 0 Reviews / 0 Questions
$985.80

  • Please complete this captcha to continue

Stock Status: In Stock

  • Please complete this captcha to continue

    * Please log into your account to avoid entering this information every time.


ITEM #: 3527818
HiTrap rProtein A FF columns are prepacked with rProtein A Sepharose Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites. The recombinant protein A ligand (produced in E. coli ) is coupled to Sepharose Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.
• Quick, convenient...
ITEM #: 3527818
HiTrap rProtein A FF columns are prepacked with rProtein A Sepharose Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites. The recombinant protein A ligand (produced in E. coli ) is coupled to Sepharose Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.
• Quick, convenient preparative purification of monoclonal antibodies. • Well-established protein A-based medium used in many approved MAb processes. • Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification. • Simple operations with a syringe, pump, or high-performance chromatography system, such as AKTA design.
.
HiTrap is a registered trademark of Cytiva
Sepharose is a registered trademark of Cytiva
Read More >>

Cytiva - 17507901 - HiTrap rProtein A FF, 5 x 1 mlItem must be purchased in units of 1

  • Item #: 3527818
  • MFR SKU: 17507901
Technical Specs
  • shelf life :Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
  • packaging :pack of 5 × 1 mL
  • parameter :< 4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
  • :42 psi
  • bed size :7 mm × 25 mm
  • bed volume :1 mL
  • column I.D. :7 mm
  • matrix :4% cross-linked agarose
  • particle size :60-165 μm
  • average diameter :90 μm (d50v)
  • cleaning :2 - 11 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
  • working range :3 - 10
  • capacity :~50 mg binding capacity (human lgG/ml medium)(Capacity of Hi. Trap r. Protein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M Na. Cl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.)
  • suitability :suitable for bioprocess medium
  • storage temp. :2-8° C
Questions & Answers

There are no questions about this product yet. Be the first to ask a question.

Add Question
0 Customer Ratings & Reviews
0 out of 5 stars
5 Star
0 %
4 Star
0 %
3 Star
0 %
2 Star
0 %
1 Star
0 %

Review this product

Tell us about your personal experience

Write a review

Recently Viewed Items