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Cytiva - 17513203 - Glutathione Sepharose 4 Fast Flow, 500 mLItem must be purchased in units of 1

  • MFR SKU: 17513203
  • ITEM #: 3522718
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ITEM #: 3522718
Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed.
  • Fast, one-step GST-tagged protein purification.
  • Mild elution conditions preserve protein antigenicity and function.
  • Excellent scale-up due to high flow properties.
Resin for GST-tagged protein purification

Glutathione Sepharose 4 Fast Flow is used to purify glutathione S-transferase (GST)-tagged...
ITEM #: 3522718
Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed.
  • Fast, one-step GST-tagged protein purification.
  • Mild elution conditions preserve protein antigenicity and function.
  • Excellent scale-up due to high flow properties.
Resin for GST-tagged protein purification

Glutathione Sepharose 4 Fast Flow is used to purify glutathione S-transferase (GST)-tagged proteins from bacterial lysates. It is also suitable for purifying other S-transferases or glutathione-dependent proteins. Successful use in published research

Glutathione Sepharose 4 Fast Flow resin is used in a variety of applications, including:
  • Characterization of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases
  • Apical sorting of lysoGPI-anchored proteins independent ofassociation with detergent-resistant membranes
  • Structure and function of the Rad9-binding region of theDNA-damage checkpoint adaptor TopBP1
Compatible empty columns

Life Sciences solutions from include a variety of empty columns for packing your own columns. Glutathione Sepharose 4 Fast Flow is compatible for use with gravity, HiScale, Tricorn, and XK columns.Convenient prepacked columns

Glutathione Sepharose 4 Fast Flow resin is also available in the following prepacked formats:
  • GSTrap FF: HiTrap format for convenient use with a syringe, a pomp, or a chromatography system
  • GSTPrep FF: for convenient scale-up
Other glutathione agarose products from

This listing is for package of 500 mL.
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Cytiva - 17513203 - Glutathione Sepharose 4 Fast Flow, 500 mLItem must be purchased in units of 1

  • Item #: 3522718
  • MFR SKU: 17513203
Technical Specs
  • Flow velocity: < 450 cm/h
  • Ligand: Glutathione and 10-carbon linker arm
  • Particle size, d 50V : ~90 µm ( Median particle size of the cumulative volume distribution. )
  • Matrix: Highly cross-linked 4% agarose
  • Ligand Coupling Method: Epoxy activation
  • Binding Capacity/ml Chromatography Medium: >10 mg/mL ( Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution. )
  • pH stability, operational: 3–12 ( pH range where resin can be operated without significant change in function. )
  • pH stability, CIP: 3–12 ( pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function. )
  • Pressure/Flow Specification: base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
  • Storage: 4 to 30°C, 20% Ethanol
  • Chemical stability: Commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature
  • Licensing: For licensing information, see the Licensing Statements page in the About Us section.
  • Chromatography technique: GST-tagged protein purification
  • Pack size: 500 mL
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