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Cytiva - 17515501 - HiTrap CM FF Item must be purchased in units of 1

  • MFR SKU: 17515501
  • ITEM #: 3527825
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ITEM #: 3527825
HiTrap CM FF is prepacked with CM Sepharose Fast Flow, and is a weak cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.
  • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  • The industry standard for ion exchange chromatography.
  • High flow rates and good scale-up potential.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • Predictable...
ITEM #: 3527825
HiTrap CM FF is prepacked with CM Sepharose Fast Flow, and is a weak cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.
  • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  • The industry standard for ion exchange chromatography.
  • High flow rates and good scale-up potential.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • Predictable scale-up

Q, SP, DEAE adn CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a resin with higher porosity, which is particularly useful for the purification of high molecular mass proteins.
This listing is for package of 5 × 5 mL.
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Cytiva - 17515501 - HiTrap CM FF Item must be purchased in units of 1

  • Item #: 3527825
  • MFR SKU: 17515501
Technical Specs
  • Bed Volume: 5 ml
  • Storage Conditions: 4 to 30°C, 20% Ethanol
  • Pressure Max. [Over the Packed Bed During Operation]: 5 bar [0.5 MPa] (70 psi)
  • Application: For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules
  • BioProcess resin: Yes
  • Ligand: Carboxymethyl
  • Particle size, d50V: ~90 µm1)
  • Ion Exchanger Type: Weak cation exchanger
  • Matrix: cross-linked agarose, 6%, spherical
  • Ionic Capacity: 0.09-0.13 mmol H+/ml medium
  • PH stability, operational: 4-132)
  • PH stability, CIP: 2-143)
  • PH ligand fully charged: Above 64)
  • Pressure/Flow Specification: 300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm
  • Exclusion Limit [Mr] [Globular Proteins]: ~4 × 106
  • Storage: 4 to 30°C, 20% Ethanol
  • Chemical Stability: Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol5)
  • Column i.d.: 16 mm
  • Bed Dimensions: 16 × 25 mm
  • Complete Packsize: 5 ml
  • Material [Column Hardware]: Polypropylene (PP)
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