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Cytiva - 17543203 - Butyl Sepharose High Performance, 1 LItem must be purchased in units of 1

  • MFR SKU: 17543203
  • ITEM #: 16918483
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Average lead time for this brand: 2-3 weeks. Specific products may vary.

ITEM #: 16918483
Butyl Sepharose High Performance is an aliphatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority
  • Aliphatic HIC resin designed for intermediate purification and polishing steps
  • Selectivity similar to Butyl Sepharose 4 Fast Flow but higher resolution due to the smaller 34 µm bead size
  • Relatively high ligand density for high dynamic binding capacity even at relatively...
ITEM #: 16918483
Butyl Sepharose High Performance is an aliphatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority
  • Aliphatic HIC resin designed for intermediate purification and polishing steps
  • Selectivity similar to Butyl Sepharose 4 Fast Flow but higher resolution due to the smaller 34 µm bead size
  • Relatively high ligand density for high dynamic binding capacity even at relatively low salt concentrations
  • BioProcess resin supported for industrial applications
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding.

Butyl Sepharose High Performance is a member of the range of HIC resins for intermediate purification and polishing of proteins. Butyl Sepharose High Performance is based on highly crosslinked, 34-µm agarose beads modified with aliphatic butyl groups via uncharged, chemically stable ether linkages. It is a truly hydrophobic resin displaying a minimum of ionic interactions. Butyl Sepharose High Performance resins is particularly well suited for intermediate purification and polishing steps providing high resolution due to the small particle size.
Butyl Sepharose High Performance is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess resins range, Butyl Sepharose High Performance meets industrial demands with security of supply and comprehensive technical and regulatory support
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Cytiva - 17543203 - Butyl Sepharose High Performance, 1 LItem must be purchased in units of 1

  • Item #: 16918483
  • MFR SKU: 17543203
Technical Specs
  • Particle Size: 24 µm-44 µm
  • Bio. Process resin: Yes
  • Ligand: Butyl
  • Particle size, d 50V : ~34 µm ( Median particle size of the cumulative volume distribution. )
  • Matrix: cross-linked agarose
  • Ligand density: Approx. 50 µmol butyl/ml gel
  • pH stability, operational: 3–13 ( pH range where resin can be operated without significant change in function. )
  • pH stability, CIP: 2–14 ( pH range where resin can be subjected to cleaning- or sanitization-in-place without significant change in function. )
  • Pressure/Flow Specification: Base matrix: 100-200 cm/h, 300 k. Pa, Bio. Pilot 60/600 column, bed height 30 cm
  • Storage: 4 to 30°C, 20% Ethanol
  • Chemical stability: Stable in commonly used aqueous buffers - 1 M sodium hydroxide, 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% acetonitrile, 70% ethanol, 3 M ammoniumsulfate, 1 mM HCl, 30% isopropanol, 2% SDS
  • Pack size: 1 L
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