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Cytiva - 17547851 - HiTrap Protein L, 5 × 1 mlItem must be purchased in units of 1

  • MFR SKU: 17547851
  • ITEM #: 9957458
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ITEM #: 9957458

Want to learn about our latest protein L resin? Check out Hi. Trap column with Mab. Select VL resin, with higher binding capacity and more robust alkaline stability than Capto L resins.
Hi. Trap Protein L columns are prepacked with Capto L affinity chromatography resin for purification of antibody fragments containing the kappa light chain.
  • Suitable for the capture of a wide range of antibody fragments such as antigen-binding fragments (Fabs), domain antibodies (d. Abs), and...
ITEM #: 9957458

Want to learn about our latest protein L resin? Check out Hi. Trap column with Mab. Select VL resin, with higher binding capacity and more robust alkaline stability than Capto L resins.
Hi. Trap Protein L columns are prepacked with Capto L affinity chromatography resin for purification of antibody fragments containing the kappa light chain.
  • Suitable for the capture of a wide range of antibody fragments such as antigen-binding fragments (Fabs), domain antibodies (d. Abs), and single-chain variable fragments (sc. Fvs).
  • Enables a platform approach to antibody fragment purification.
  • High binding capacity, low ligand leakage, and selectivity for a broad range of antibody fragments reduces process time and amount of resin.
  • Convenient for process development, as well as small-scale purification.
  • Hi. Trap format enables easy connection to a syringe, peristaltic pump, or chromatography system such as an ÄKTA system.
Protein L chromatography column

Hi. Trap Protein L column is packed with Capto L resin. This resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains.
The high binding capacity and affinity to a broad range of antibody fragments makes this resin an excellent capture platform for antibody fragments containing kappa light chains. Common uses include domain antibody, sc. Fv, and Fab purification.Successful use in published research

Hi. Trap Protein L is used in a variety of research applications, including:
  • Characterization of a human colorectal cancer MDR1/P-gp Fab
  • Purification optimization of an sc. Fv against type 1 insulin-like growth factor receptor (IGF-1R)
Other Capto L chromatography columns and formats

Capto L resin is also available in the following formats:
  • Bulk Capto L resin
  • Hi. Screen Capto L prepacked column, for method and process development
  • Pre. Dictor Capto L 96-well plate for high-throughput screening or process development.

This listing is for package of 5 × 1 mL.
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Cytiva - 17547851 - HiTrap Protein L, 5 × 1 mlItem must be purchased in units of 1

  • Item #: 9957458
  • MFR SKU: 17547851
Technical Specs
  • Storage Conditions: 2°C to 8°C in 20% ethanol
  • Pressure Max. [Over the Packed Bed During Operation]: N/A (N/A)
  • BioProcess resin: Yes
  • Ligand: Recombinant protein L (E. coli), mammalian free
  • Particle size d(50v): 85 µm1)
  • Matrix: Rigid, highly cross-linked agarose
  • PH Stability Working Range: 2-N/A
  • PH Stability Ligand Dependent: N/A-N/A
  • Coupling Chemistry: Epoxy activation
  • Dynamic Capacity: Approx 25 mg human Fab /ml medium2)
  • Recommended Cleaning: 15 mM NaOH
  • Bed Dimensions: 7 mm × N/A
  • Column i.d.: 7 mm
  • Complete Packsize: 1 ml
  • Material [Column Hardware]: Polypropylene (PP)
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