Image is an illustration, may not reflect the exact product sold.


Other products by Cytiva

Cytiva - 27084601 - THROMBIN PROTEASE500U F/SITE SPEF SEPN (Each)

  • MFR SKU: 27084601
  • ITEM #: 3019963
    • 0 out of 5 stars
    • 5 Star
      0 %
    • 4 Star
      0 %
    • 3 Star
      0 %
    • 2 Star
      0 %
    • 1 Star
      0 %
  • 0 Reviews / 0 Questions
$256.33

  • Please complete this captcha to continue

Stock Status: In Stock

  • Please complete this captcha to continue

    * Please log into your account to avoid entering this information every time.


ITEM #: 3019963
Thrombin is a serine protease purified from bovine plasma for digestion of GST-tagged proteins or other tagged proteins that contain the thrombin recognition sequence.
  • Specific cleavage - enables site-specific cleavage of fusion proteins containing an accessible recognition sequence.
  • Functionally free of other clotting factors such as plasminogen and plasmin.
Thrombin protease for fusion protein cleavage

Thrombin is used to digest fusion proteins prepared...
ITEM #: 3019963
Thrombin is a serine protease purified from bovine plasma for digestion of GST-tagged proteins or other tagged proteins that contain the thrombin recognition sequence.
  • Specific cleavage - enables site-specific cleavage of fusion proteins containing an accessible recognition sequence.
  • Functionally free of other clotting factors such as plasminogen and plasmin.
Thrombin protease for fusion protein cleavage

Thrombin is used to digest fusion proteins prepared from pGEX vectors containing the recognition sequence for thrombin. The following vectors encode that sequence: pGEX-1λT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3.
thrombin protease is “protein friendly, ” preserving protein structure and stability throughout purification to maximize samples and results. The product also streamlines methodology, allowing users to detect expressed proteins by direct labeling of fusion products in vitro.Successful use in published research

Thrombin protease from is used for GST cleavage in a variety of research applications, including:
  • Dissociation of Endos from beads during study of protein phosphatase 2A in mitosis
  • Purification of LR11 transmembrane and cytoplasmic domains during study of human APP binding protein LR11/SorLA for NMR studies

This listing is for package of 500 units.
Read More >>

Cytiva - 27084601 - THROMBIN PROTEASE500U F/SITE SPEF SEPN (Each)

  • Item #: 3019963
  • MFR SKU: 27084601
Technical Specs
  • Application: Used for site-specific separation of the GST tag from proteins expressed using pGEX-T vectors
  • Cleavage Buffer: PBS: 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.3
  • Complete Packsize: 500 units
  • Elution Buffer: 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0
  • Inhibitor: Pefabloc TH benzamidine, AEBSF, APMSF, antithrombin III, Antipain, ?1-antitrypsin, aprotinin, chymostatin, hirudin, leupeptin, PMSF
  • Molecular Weight [Mr]: 37000
  • Supplied As: Lyophilized powder
  • Unit Definition: One unit of enzyme cleaves 90% of 100 ?g of a test GST fusion protein when incubated in 1 PBS at 22C for 16 h.
  • Vector: pGEX-4T-1, pGEX-4T-2, pGEX-4T-3, pGEX-2TK
Questions & Answers

There are no questions about this product yet. Be the first to ask a question.

Add Question
0 Customer Ratings & Reviews
0 out of 5 stars
5 Star
0 %
4 Star
0 %
3 Star
0 %
2 Star
0 %
1 Star
0 %

Review this product

Tell us about your personal experience

Write a review

Recently Viewed Items