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Cytiva - 28400990 - His MultiTrap FFItem must be purchased in units of 1

  • MFR SKU: 28400990
  • ITEM #: 3527917
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$1,263.00
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ITEM #: 3527917
His Multi. Trap â„¢ FF and His Multi. Trap â„¢ HP are prepacked disposable 96-well plates for reproducible high throughput parallel purification of histidine-tagged recombinant proteins by Immobilized Metal ion Affinity Chromatography (IMAC).
His Multi. Trap â„¢ FF and His Multi. Trap â„¢ HP are prepacked 96-well filter plates for high-throughput screening of histidine-tagged* Proteins from clarified or unclarified lysates. Purification is performed by immobilized metal ion...
ITEM #: 3527917
His Multi. Trap â„¢ FF and His Multi. Trap â„¢ HP are prepacked disposable 96-well plates for reproducible high throughput parallel purification of histidine-tagged recombinant proteins by Immobilized Metal ion Affinity Chromatography (IMAC).
His Multi. Trap â„¢ FF and His Multi. Trap â„¢ HP are prepacked 96-well filter plates for high-throughput screening of histidine-tagged* Proteins from clarified or unclarified lysates. Purification is performed by immobilized metal ion affinity chromatography (IMAC). The plates are prepacked with Ni Sepharose 6 Fast FLow (His Multi. Trap â„¢ FF) or Ni Sepharose High Performance (His Multi. Trap â„¢ HP). A protocol for His Multi. Trap â„¢ is available in His Buffer Kit. The kit is available with ready-made buffer solutions, for increased convenience and for fast buffer preparation. Loading unclarified samples directly to His Multi. Trap â„¢ FF and His Multi. Trap â„¢ HP simplifies and shortens the overall time for expression screening, which minimizes degradation and oxidation of sensitive target Proteins. His Multi. Trap â„¢ FF and His Multi. Trap â„¢ HP prepacked filter plates are manufactured with high reproducibility with regards to the well volume and the volume of medium to give consistent results.
• Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged* Proteins. • Increased convenience and consistency with prepacked 96-well plates. • No filtration needed - load unclarified sample directly, increase reproducibility, and save time. • Use His Multi. Trap ™ 96-well filter plates with robotic systems or manually with centrifugation or vacuum. • Easy scale-up to His. Trap ™ FF, His. Prep ™ FF 16/10 or His. Trap ™ HP prepacked columns. High chemical stability and high binding capacity up to 1 mg of histidine-tagged Protein per well.
Store at 4 to 30 °C (20% Ethanol)
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His. Prep is a trademark of Cytiva
His. Trap is a trademark of Cytiva
Multi. Trap is a trademark of Cytiva
Sepharose is a registered trademark of Cytiva
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Cytiva - 28400990 - His MultiTrap FFItem must be purchased in units of 1

  • Item #: 3527917
  • MFR SKU: 28400990
  • Chromatography Medium: Ni Sepharose 6 Fast Flow
  • Complete Packsize: 4 x 96-well filter plates
  • Centrifugation Force Max.: 700 x g
  • Storage Conditions: 4 to 30°C, 20% Ethanol
  • BioProcess resin: Yes
  • Particle size, d50V: 90 µm1)
  • Matrix: 6% cross-linked agarose
  • Binding Capacity/ml Chromatography Medium: Approx. 40 mg histidine-tagged protein
  • Metal Ion Capacity: ~15 µmol Ni2?/ml medium
  • PH Stability Working Range: 3-122)
  • PH stability, CIP: 2-143)
  • Pressure/Flow Specification: Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
  • Chemical Stability: Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea4)
  • Number of Wells: 96
  • Sample Volume: 70-130 ?l
  • Reproducibility Between Wells: ± 10% in yield
  • Material: Polypropylene (PP) and polyethylene (PE).
  • Licensing: For licensing information, see the Licensing Statements page in the About Us section.

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