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Cytiva - 29148723 - Superdex 75 Increase 3.2/300Item must be purchased in units of 1

  • MFR SKU: 29148723
  • ITEM #: 13588095
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ITEM #: 13588095
Superdex 75 Increase 10/300 GL, 5/150 GL, and 3.2/300 are prepacked columns for versatile use in size exclusion chromatography applications. The columns are intended for use in preparative purification as well as for characterization and analysis of proteins with molecular weights between M r 3000 and 70 000, such as recombinant tagged proteins.
  • Up to 50% higher resolution compared with predecessor Superdex 75, for improved purity and analysis results.
  • Runtime reduced down to...
ITEM #: 13588095
Superdex 75 Increase 10/300 GL, 5/150 GL, and 3.2/300 are prepacked columns for versatile use in size exclusion chromatography applications. The columns are intended for use in preparative purification as well as for characterization and analysis of proteins with molecular weights between M r 3000 and 70 000, such as recombinant tagged proteins.
  • Up to 50% higher resolution compared with predecessor Superdex 75, for improved purity and analysis results.
  • Runtime reduced down to one third compared with predecessor Superdex 75, with maintained resolution for results faster.
  • Versatile use in both preparative and analytical applications, especially with recombinant tagged proteins.
  • Higher lot-to-lot consistency compared with predecessor Superdex 75.
  • Tolerance to repeated harsh cleaning procedures at high pH ensures long column lifetime and minimizes carryover.
Higher resolution and/or reduced runtime compared with predecessor

Superdex 75 Increase belongs to the new generation of agarose based SEC media, comprising smaller, more rigid beads with a narrower particle size distribution and a higher selectivity for improved performance compared with its predecessor Superdex 75. Clean in place for versatile use

Superdex 75 Increase resin is alkali-resistant. Hence, the column can be cleaned in place under alkali conditions to allow use of the same column for different proteins with minimal risk for carryover between samples. The alkali resistance is what differentiates agarose-based resins from silica-based resins. The possibility to clean the resin under harsh alkali conditions also ensures a long column lifespan.
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Cytiva - 29148723 - Superdex 75 Increase 3.2/300Item must be purchased in units of 1

  • Item #: 13588095
  • MFR SKU: 29148723
Technical Specs
  • Bed dimensions: 3.2 × 300 mm
  • Bed height: 300 mm
  • Bed support: Frit
  • Bed volume: ~ 2.4 mL
  • Chemical stability: Conditions where the resin may be operated without significant change in function: • All commonly used aqueous buffers, pH 3 to 12
  • • Urea, up to 8 M
  • • Ionic and nonionic detergents, e.g., 1% SDS
  • • Guanidine hydrochloride, up to 6 M
  • • Acetic acid, up to 1 M
  • • Methanol, up to 10%
  • • Isopropanol, up to 5%
  • • Dithiothreitol (DTT), up to 5 mM
  • • Sodium hydroxide, up to 0.5 M
  • Conditions where the resin can be subjected to cleaning- or sanitization-in-place without significant change in function: • Acetonitrile, up to 30%
  • • Sodium hydroxide, up to 1 M
  • • Ethanol, up to 70%
  • • Methanol, up to 70%
  • • Isopropanol, up to 30%
  • • Hydrochloric acid, up to 0.1 M
  • • Trifluoroacetic acid, up to 1%
  • • Formic acid, up to 70%
  • Avoid: • Oxidizing agents
  • • Unfiltered samples and eluents
  • Chemical stability (column hardware): Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.
  • Column i.d.: 3.2
  • Exclusion limit (M r ) (globular proteins): ~ 1 × 10 5
  • Fractionation range (M r ) (globular proteins): 3000 to 70 000
  • Material (column hardware): Borosilicate glass, polyetheretherketone (PEEK), polypropylene (PP), ethylene propylene diene monomer (EPDM), and perfluoro-rubber (PFR)
  • Material (bed support): Titanium
  • Material (column tube): Borosilicate glass
  • Matrix: Composite of cross-linked agarose and dextran
  • Operating flow rate, max.: 0.15 mL/min, water at room temperature;
  • 0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
  • 0.075 mL/min water at low temperature;
  • 0.035 mL/min 20% ethanol or 10% glycerol at low temperature
  • Operating flow rate, recommended: 0.075 mL/min ( Water at 20?C to 25?C. )
  • Particle size, d 50V : ~ 9 µm ( Median particle size of the cumulative volume distribution. )
  • Sample volume: 4 to 50 µL
  • Storage conditions: 4?C to 30?C, 20% ethanol
  • Theoretical plates: > 43 000 m -1
  • Typical pressure drop over the packed bed: ~ 2 MPa ( At 25?C in water. Determine the individual column limit according to product instructions for use )
  • pH stability, cleaning-in-place (CIP): 1 to 14
  • pH stability, operational: 3 to 12
  • Bed Dimensions: 3.2 × 300 mm
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