Image is an illustration, may not reflect the exact product sold.

Cytiva - 29219758 - Superdex 30 Increase 3.2/300Item must be purchased in units of 1

MFR SKU: 29219758

ITEM #: 13592763

- 0 out of 5 stars
- 5 Star
  0 %
- 4 Star
  0 %
- 3 Star
  0 %
- 2 Star
  0 %
- 1 Star
  0 %
0 Reviews / 0 Questions

Lists At: $3,460.57 You Save: $229.57

$3,231.00

Saw It For Less?

Stock Status: Low Stock

ITEM #: 13592763
Superdex 30 Increase SEC columns are for high resolution, small-scale purification and analysis of small biomolecules with M r ~ 100 to ~ 7000, such as peptides

Improved performance improved resolution and runtime compared to predecessor Superdex Peptide
Tolerance to repeated harsh cleaning procedures at high pH ensures long column lifetime and minimizes carryover.
Very high resolution small bead size and narrow particle size distribution provide high resolution,...

ITEM #: 13592763
Superdex 30 Increase SEC columns are for high resolution, small-scale purification and analysis of small biomolecules with M r ~ 100 to ~ 7000, such as peptides

Improved performance improved resolution and runtime compared to predecessor Superdex Peptide
Tolerance to repeated harsh cleaning procedures at high pH ensures long column lifetime and minimizes carryover.
Very high resolution small bead size and narrow particle size distribution provide high resolution, for high protein purity.
High flow rate due to rigid beads giving excellent pressure/flow properties.

New generation of size exclusion chromatography columns

Superdex 30 Increase is part of Cytiva''s new generation of agarose-based SEC columns called “Increase”, which offer higher resolution purification in shorter runtimes compared with their predecessors. SEC columns for analysis and purification of peptides

Superdex 30 Increase is suitable for both preparative and analytical purposes.
The fractionation range allows preparative separation of peptides, oligosaccharides and other small biomolecules (M r ~ 100 to ~ 7000) with sample volumes up to 500 µL.
These columns also enable protein characterization, including aggregate analysis, size homogeneity analysis, and interaction studies, where high-quality data can be achievedCleaning-in-place (CIP) capabilities

This agarose-based resin is alkali resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different biomolecules, with minimal risk of carry-over between samples.
Regular cleaning can also increase column lifetime.Two SEC column sizes

Two size exclusion chromatography (SEC) column sizes are designed to serve different purposes:

10/300 GL column, 10 mm × 300 mm, for high resolution analysis as well as for small-scale (25–500 µL) preparative purification, such as the polishing step in a purification protocol
3.2/300 column, 3.2 mm × 300 mm, for high resolution analysis and purification of sample volumes of 4–50 µl, when sample amount is limited and low buffer consumption is important
It is also possible to order Superdex 30 Increase resin to be packed in 5/150 GL column, 5 mm x 150 mm, as custom designed product.

Other size exclusion chromatography columns

offers a wide range of Life Sciences solutions for SEC (or gel filtration). Let help you find the right SEC column for your needs.

Cytiva - 29219758 - Superdex 30 Increase 3.2/300Item must be purchased in units of 1

Item #: 13592763

MFR SKU: 29219758

Technical Specs

Bed dimensions: 3.2 � 300 mm
Bed height: 300 mm
Bed support: Frit
Bed volume: ~ 2.4 mL
Chemical stability: Conditions where the resin may be operated without significant change in function: � Trifluoroacetic acid, up to 0.2%
� Isopropanol, up to 5%
� Dithiothreitol (DTT), up to 5 mM
� Acetonitrile, up to 70%
� Formic acid, up to 1%
� All commonly used aqueous buffers, pH 3 to 12
� Urea, up to 8 M
� Guanidine hydrochloride, up to 6 M
� Ionic and nonionic detergents, e.g., 1% SDS
� Methanol, up to 70%
Conditions where the resin can be subjected to cleaning- or sanitization-in-place without significant change in function: � Sodium hydroxide, up to 1 M
� Ethanol, up to 70%
� Isopropanol, up to 30%
� Hydrochloric acid, up to 0.1 M
� Trifluoroacetic acid, up to 1%
� Dimethyl sulfoxide (DMSO), up to 10%
Avoid: � Oxidizing agents
� Unfiltered samples and eluents

Chemical stability (column hardware): Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.
Column i.d.: 3.2
Fractionation range (M r ) (globular proteins): 100 to 7000
Material (column hardware): Borosilicate glass, polyetheretherketone (PEEK), polypropylene (PP), ethylene propylene diene monomer (EPDM) and perfluoro-rubber (PFR)
Material (bed support): Titanium
Material (column tube): Borosilicate glass
Matrix: Composite of cross-linked agarose and dextran
Operating flow rate, max.: 0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature
Operating flow rate, recommended: 0.075 mL/min ( Water at 20?C to 25?C. )
Particle size, d 50V : ~ 9 �m ( Median particle size of the cumulative volume distribution. )
Sample volume: 4 to 50 �L
Storage conditions: 4?C to 30?C, 20% ethanol
Theoretical plates: > 38 000 m -1
Typical pressure drop over the packed bed: ~ 2 MPa ( At 25?C in water. Determine the individual column limit according to product instructions for use. )
pH stability, cleaning-in-place (CIP): 1 to 14
pH stability, operational: 3 to 12
Bed Dimensions: 3.2 � 300 mm

Questions & Answers

Add Question

0 Customer Ratings & Reviews

Review this product

Tell us about your personal experience

Write a review

5 Star		0 %
4 Star		0 %
3 Star		0 %
2 Star		0 %
1 Star		0 %

Cytiva - 29219758 - Superdex 30 Increase 3.2/300Item must be purchased in units of 1

Cytiva - 29219758 - Superdex 30 Increase 3.2/300Item must be purchased in units of 1

Technical Specs

Questions & Answers

0 Customer Ratings & Reviews

Review this product

Recently Viewed Items