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Cytiva - E70073X - Exonuclease IItem must be purchased in units of 1

  • MFR SKU: E70073X
  • ITEM #: 3525453
  • 0 Reviews / 0 Questions
$1,020.00
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ITEM #: 3525453

Exonuclease I is used to selectively digest excess single stranded DNA in a mixture also containing double-stranded DNA, such as removing unused primers after PCR.
  • 3'5' single-stranded exonuclease.
  • Monomeric 54.5 kDa bacterial exonuclease I recombinantly expressed in E. coli .
  • Inactivated by heating at 80°C for 15 minutes.
  • Active in PCR buffers.

Exonuclease I, used together with Alkaline Phosphatase for inactivation of dNTPs,...
ITEM #: 3525453

Exonuclease I is used to selectively digest excess single stranded DNA in a mixture also containing double-stranded DNA, such as removing unused primers after PCR.
  • 3'5' single-stranded exonuclease.
  • Monomeric 54.5 kDa bacterial exonuclease I recombinantly expressed in E. coli .
  • Inactivated by heating at 80°C for 15 minutes.
  • Active in PCR buffers.

Exonuclease I, used together with Alkaline Phosphatase for inactivation of dNTPs, provides effective cleanup of PCR reactions for subsequent use in DNA sequencing.
Digestion of single stranded DNA by Exonuclease I releases dNTPs leaving 5'-terminal dinucleotides intact. DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups are not removed.
This listing is for package of 5000 units.
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Cytiva - E70073X - Exonuclease IItem must be purchased in units of 1

  • Item #: 3525453
  • MFR SKU: E70073X
  • Activity: 10 units/µl
  • Purity: Free of contaminating endonucleases, double-stranded exonucleases and ribonucleases. Greater than 95% pure as determined by SDS-PAGE
  • Pack size: 5000 units

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