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Cytiva - E79000Z - Thermo Sequenase DNA Polymerase (with Thermoplasma acidophilum Inorganic Pyrophosphatase (TAP)) Item must be purchased in units of 1

  • MFR SKU: E79000Z
  • ITEM #: 9628295
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ITEM #: 9628295

Thermo Sequenase DNA polymerase is a novel thermostable DNA polymerase that uses ddNTPs as readily as dNTP substrates at temperatures similar to those used in standard PCR. The result is:
  • Uniform and easy-to-read band patterns
  • Better incorporation of ddNTPs helps avoid secondary structures that can cause unreadable sequences
  • Read-through of difficult sequences (e.g., GC-rich), due to the ability of thermostable enzymes to function at elevated temperature used for sequencing
...
ITEM #: 9628295

Thermo Sequenase DNA polymerase is a novel thermostable DNA polymerase that uses ddNTPs as readily as dNTP substrates at temperatures similar to those used in standard PCR. The result is:
  • Uniform and easy-to-read band patterns
  • Better incorporation of ddNTPs helps avoid secondary structures that can cause unreadable sequences
  • Read-through of difficult sequences (e.g., GC-rich), due to the ability of thermostable enzymes to function at elevated temperature used for sequencing

ddNTPs are chain-elongating inhibitors of DNA polymerase, which are used in the Sanger method for DNA sequencing. Each nucleotide base of that particular type has a probability of being bonded not to a deoxynucleotide but rather a dideoxynucleotide, which ends chain elongation. This property, in addition to thermostability, makes Thermo Sequenase DNA polymerase an excellent choice for cycle sequencing where template quantity limitations preclude the use of T7 Sequenase DNA polymerase.
The properties of Thermo Sequenase also enable its use in many SNP genotyping, genotyping by sequencing, or genome sequencing applications where primer extension is employed.
DNA polymerases such as Thermo Sequenase can be incorporated into custom-made cycle sequencing kits, using our custom manufacturing facilities .
This listing is for package of 10000 units.
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Cytiva - E79000Z - Thermo Sequenase DNA Polymerase (with Thermoplasma acidophilum Inorganic Pyrophosphatase (TAP)) Item must be purchased in units of 1

  • Item #: 9628295
  • MFR SKU: E79000Z
Technical Specs
  • Assay Conditions: 25 mM TAPS buffer, pH 9.3 (at 25° C), 50 mM KCl, 2 mM MgCl2, 1 mM 2-mercaptoethanol, 200 µm dATP, dGTP, dTTP, 100 µ M [a-32P]-dCTP (0.05-0.1 Ci/mmol), and 400 µg/ml activated DNA. After incubation at 74° C for 10 min
  • Storage conditions: -20° C, Solution in 20 mM Tris-HCl, pH 8.5, 0.1 mM EDTA, 0.5% Tween 20 (v/v), 0.5% Nonidet P-40 (v/v), 1 mM DTT, 100 mM KCl, 50% glycerol
  • Components: Thermo Sequenase™/TAP; Dilution buffer; Reaction Buffer
  • Activity: 32 units/µl
  • Application: DNA Sequencing SNP genotyping applications where primer extension is employed
  • Functional testing: Fluorescent 4-dye primers used to sequence single-or double-stranded DNA templates. Accuracy of 98.5% up to 500 base pairs is obtained using an Applied Biosystems model 373 stretch DNA Sequencer using a 34 cm well-to-read distance
  • Licensing: For licensing information, see the Licensing Statements page in the About Us section.
  • Source: E. coli clones that overproduce the protein
  • Unit definition: One unit of enzyme incorporates 10 nmol of dNTPs into acid-insoluble material in 30 min at 74° C
  • Pack size: 10000 units
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