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Cytiva - RPN2108 - Amersham ECL Western Blotting Detection KitItem must be purchased in units of 1

  • MFR SKU: RPN2108
  • ITEM #: 3020646
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ITEM #: 3020646
Each kit contains the detection reagent, HRP conjugated antibodies, and blocking agent sufficient for detection of 1000 cm² membrane.
  • Detection of mouse and rabbit primary antibodies on western blots using HRP-conjugated secondary antibodies and ECL reagents.
  • Optimized system provides rapid, sensitive results using simple protocols.
  • Designed for use with nitrocellulose and PVDF membranes.
  • High sensitivity: Up to 10 times more sensitive than colorimetric methods.
  • Rapid:...
ITEM #: 3020646
Each kit contains the detection reagent, HRP conjugated antibodies, and blocking agent sufficient for detection of 1000 cm² membrane.
  • Detection of mouse and rabbit primary antibodies on western blots using HRP-conjugated secondary antibodies and ECL reagents.
  • Optimized system provides rapid, sensitive results using simple protocols.
  • Designed for use with nitrocellulose and PVDF membranes.
  • High sensitivity: Up to 10 times more sensitive than colorimetric methods.
  • Rapid: Results can be rapidly obtained on X-ray film, providing hard copy data and avoiding fading associated with colorimetric detection methods.
  • Easy reprobing: Save time and materials by reprobing on membranes up to 10 times.
  • Quantifiable: Linear response in the range 0.2 to 2 OD units using pre-flashed Hyperfilm ECL,
  • Non-radioactive-no specialized facilities needed
  • Proven performance-the most widely referenced chemiluminescent detection method with hundreds of publications worldwide

Amersham ECL Western Blotting Detection Kit uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL Detection Reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera.
ECL Western Blotting System is the world’s most widely used and referenced chemiluminescent immunodetection system.
Each system contains the following reagents, sufficient for detection of 1000 cm² membrane:Anti-mouse HRP conjugate, 100 µlAnti-rabbit HRP conjugate, 100 µlECL Detection Reagents 1 and 2, 62.5 ml eachBlocking agent, 5 g.
This listing is for package of For detecting 1000 cm².
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Cytiva - RPN2108 - Amersham ECL Western Blotting Detection KitItem must be purchased in units of 1

  • Item #: 3020646
  • MFR SKU: RPN2108
Technical Specs
  • Components: The pack contains the following reagents: 100 µl Anti-mouse HRP conjugate 100 µl Anti-rabbit HRP conjugate 62.5 ml ECL detection reagent 1 62.5 ml ECL detection reagent 2 5 g Blocking agent Sufficient for 10 blots (10 cm×10 cm).
  • Guidance: Stability: The components of this product are stable until expiration date printed on box when stored under recommended conditions.
  • Wetted Part: No
  • Application: For research use only
  • Color: Detection reagent 1: Colorless Detection reagent 2: Colorless Peroxidase labelled anti-mouse antibody: Colorless Peroxidase labelled anti-rabbit antibody: Colorless Blocking agent: White
  • Recommended Membrane: Nitrocellulose, PVDF membranes
  • Solubility: Detection reagent 1: Soluble in cold water and hot water Detection reagent 2: Soluble in cold water and hot water Peroxidase labelled anti-mouse antibody: Soluble in cold water, hot water, methanol, acetone Peroxidase labelled anti-rabbit antibody: Soluble in cold water, hot water, methanol, acetone
  • Storage conditions: 2 - 8°C
  • Pack size: For detecting 1000 cm²
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