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Daigger - EF21417 - Daigger X-GAL

  • MFR SKU: EF21417
  • ITEM #: 23376967
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ITEM #: 23376967
X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL resulted in the production of water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13amp18 and 19, PUC18 and 19, Pur222 the E.coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing...
ITEM #: 23376967
X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL resulted in the production of water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13amp18 and 19, PUC18 and 19, Pur222 the E.coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells product a blue indigo dye.
 
Soluble in DMSO and DMF. Store at 2-8°C. [5-Bromo-$chloro-3-indolyl-β-D-galactoside]
 Key Features
  • CAS No. 7240-90-6
  • C 14 H 15 BrCINO 6
  • MW 408.6
  • Purity >99%
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Daigger - EF21417 - Daigger X-GAL

  • Item #: 23376967
  • MFR SKU: EF21417
  • Attribute Description: X-GAL; 50 g, Unit of Measure: Each

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