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FD NeuroTechnologies - PK201 - FD NeuroApop Kit

  • MFR SKU: PK201
  • ITEM #: 12957751
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ITEM #: 12957751
FD NeuroApop Kit is specifically designed for the detection of neuronal apoptosis in tissue sections from the central nervous system based on the principle of in situ DNA nick-end labeling (TUNEL) technique. The assay uses terminal deo x ynucleotidyl transferase to catalyze the incorporation of biotinylated deo x yuridines onto the free 3'-hydro x yl termini of DNA fragments, which are considered one of the most characteristic features of apoptosis. The integrated biotins are amplified and visualized...
ITEM #: 12957751
FD NeuroApop Kit is specifically designed for the detection of neuronal apoptosis in tissue sections from the central nervous system based on the principle of in situ DNA nick-end labeling (TUNEL) technique. The assay uses terminal deo x ynucleotidyl transferase to catalyze the incorporation of biotinylated deo x yuridines onto the free 3'-hydro x yl termini of DNA fragments, which are considered one of the most characteristic features of apoptosis. The integrated biotins are amplified and visualized with the avidin-biotin-comple x (ABC) method, enabling light microscopic identification.
The reagents and procedure of FD NeuroApop Kit have been optimized to achieve a high degree of both specificity and sensitivity for detecting apoptotic neurons with the lowest background. This kit can be used with frozen and paraffin sections, as well as cultured cells. The procedure of the kit takes appro x imately 4 hours.
Kit Contents:
Part I (Store at -20°C)
  • Digestive Enzyme, 2mL x 4
  • Reaction Solution A, 2mL x 2
  • Reaction Solution B, 85 µL
  • Reaction Solution C, 60 µL
  • Chromogen Solution, 20mL

Part II (Store at 4°C)
  • Equilibration Buffer, 20mL
  • Detection Reagent, 5mL
  • 10 x Phosphate-Buffered Saline, 250mL x 2

Materials required but not included:
  • Double distilled water
  • Humidified chamber
  • Incubator or waterbath (30°C)
  • Histological supplies and equipment, including microscope slides, glass coverslips, staining jars, fine-tipped forceps, ethanol, x ylenes or x ylene-substitutes, mounting medium, and a light microscope

FD NeuroApop Kit
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FD NeuroTechnologies - PK201 - FD NeuroApop Kit

  • Item #: 12957751
  • MFR SKU: PK201
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