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G-Biosciences - 786-306 - Western ReProbe [5X], 1L

  • MFR SKU: 786-306
  • ITEM #: 14275112
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ITEM #: 14275112
Western ReProbe is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe also enables the ability to reuse western blots.
Western ReProbe breaks the antigen-antibody binding affinity without using denaturants, SDS or boiling. The membrane-bound protein is retained on the membrane, and the matching antibodies are washed away. Once the antigen-antibody bonds...
ITEM #: 14275112
Western ReProbe is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe also enables the ability to reuse western blots.
Western ReProbe breaks the antigen-antibody binding affinity without using denaturants, SDS or boiling. The membrane-bound protein is retained on the membrane, and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane-bound protein is free to accept new proteins.
Removal of antibodies also removes corresponding chemiluminescent or radio-isotopic signals from the blots. The stripped blots can then be probed with new probes (see figure 1).
Western ReProbe is not recommended for stripping color-producing western blots that use substrates such as TMB, chloronapthol and DAB.
Western ReProbe is supplied as a 5X concentrated solution. Each 100 milliliters is sufficient for 25 to 30 stand ard size (7.5cm x 8.5cm) western blots.
For high affinity antibodies, we recommend our Western ReProbe PLUS.
Features
  • Simply incubate the blot in Western ReProbe at room temp & wash
  • No boiling, denaturants or SDS required
  • Repeated analysis of Western blots with multiple probes
  • Re-analysis & correction of unsatisfactory Western blots
  • Conservation of hard-to-obtain test samples & reagents
  • Time saving in not rerunning gels & transfer processes

Applications
  • Screen same blot with different antibodies
  • Reprobe for housekeeping proteins
  • Compare phosphorylated & total protein on the same blot

Western ReProbe [5X], 1L
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G-Biosciences - 786-306 - Western ReProbe [5X], 1L

  • Item #: 14275112
  • MFR SKU: 786-306
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