G-Biosciences - 786-572 - Micro Bicinchoninic Acid (BCA) Protein Assay, 500 Assays/ 3, 300 Micro-assays

ITEM #: 14275265
The Micro Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. This modification of the Bicinchoninic Acid (BCA) Protein Assay is suitable for dilute protein samples (0.5-20µg/ml).
The Micro BCA Protein Assay primarily relies on two reactions. Firstly, the peptide bonds in the protein sample reduce Cu 2+ ions, in a temperature dependent reaction, from the copper solution to Cu + . The amount of Cu 2+ reduced is proportional to the amount of protein present in the solution. Ne x t, two molecules of bicinchoninic acid chelate with each Cu + ion, forming a purple-colored product that strongly absorbs light at a wavelength of 562nm. The assay uses concentrated solutions and e x tended incubation times for the detection of dilute protein samples. The amount of protein present in a solution can be quantified by measuring the absorption spectra and comparing with protein solutions with known concentrations.
The Micro BCA Protein Assay is suitable for quantifying protein solutions in 500 x 1ml assays or in >3, 300 micro-well assays.
The assay is supplied with a traditional bovine serum albumin (BSA) protein standard or a non animal protein standard.
For samples with reducing agents, we recommend the Bicinchoninic Acid (BCA) Reducing Agent Compatible Protein Assay.
Features

• Sensitive, colorimetric assay for dilute protein samples
• Linear range: 0.5-20µg/ml
• Compatible with most non-ionic and ionic detergents

Applications

• For dilute protein samples (0.5-20µg/ml)

Micro Bicinchoninic Acid (BCA) Protein Assay, 500 Assays/ 3, 300 Micro-assays
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