G-Biosciences - 786-893 - CB Protein Assay with Non Animal Protein Standard, 500 Assays

ITEM #: 14275553
An improved Coomassie Dye based protein assay based on the Bradford Protein Assay (1). This assay is suitable for the simple and rapid estimation of protein concentration. This assay is based on a single Coomassie dye based reagent. The binding of protein to the dye results in a change of color from brown to blue. The change in color density is proportional to protein concentration. Protein estimation can be performed using as little as 0.5µg protein
CB Protein Assay is supplied with a simple to follow protocol. Ready to use reagent that does not require prefiltering or dilution. Simply mi x the protein solution with CB Protein Dye and read optical protein-dye comple x es reach a stable end point in 5 minutes. The CB Protein Assay is compatible with reducing agents and a wide variety of common laboratory agents listed below. The assay is supplied with a traditional bovine serum albumin (BSA) protein standard or a non animal protein standard.
Note: The Coomassie dye based assay is not suitable if the protein solution contains higher than recommended concentration of detergents or other agents (see Table 1). Tolerance to Common laboratory Agents
The following table kists the agents compatible with the CB Protein Assay. Table 1 also shows the acceptable concentration of reagents for standard protocols. In most cases, using a correct blank will eliminate or minimize the error caused by interference. Table 1: A selection of compounds and the ma x imum concentrations that are compatible with the CB Assay.
Compound Concentration Compound Concentration Compound Concentration
Amino acids 1mM DTT 1M Phenol 5%
Ammonium sulfate 1M EDTA 100mM Sodium azide 0.5%
Ampholytes 0.5% EGTA 50mM Sodium chloride 6M
Ascorbic acid 50mM Ethanol 10% Sodium dodecyl sulfate (SDS) 0.015%
Boric acid 1mM Glucose 1M Sodium hydro x ide 0.1M
Brij 35 0.06% Glycerol 10% Sodium phosphate 0.1M
CHAPS 0.5% Glycine 0.1M Sucrose 25%
CHAPSO 0.5% 6M Tris 2M
Citrate 0.05% HEPES 0.1M Triton X-100 0.06%
Cysteine 10mM 2-mercaptoethanol 1M tRNA 0.35mg/ml
Deo x ycholate 0.1% Methanol 10% Tween 20 0.03%
DMSO 10% MES 0.7M Urea 3M
DNA 1mg/ml Nonidet P-40 0.5%
Features

• Sensitivity: Linear responses over the range of 0.5µg-50µg protein
• Fle x ible Protocols: Suitable for tube or Titer plate assays
• Ready to use assay reagents and no preparation required
• Long shelf life, stable for 12 months

Applications

• Suitable for non-detergent solubilized proteins.
• Protein estimation in protein purification, electrophoresis, cell biology, molecular biology, and other research applications.
• Suitable for protein samples containing common laboratory agents.

CB Protein Assay with Non Animal Protein Standard, 500 Assays
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