G-Biosciences - 786-R46 - AP labeled goat a-human IgG (0.6mg/mL), 1mL

ITEM #: 10047780
We offer a range of secondary antibodies conjugated to either alkaline phosphatase (AP) or horseradish pero x idase (HRP). The antibodies are isolated from antisera by immuno-affinity chromatography using antigen coupled to sepharose beads or other methods. They are supplied lyophilized, from a buffer containing 0.01M sodium phosphate, 0.25M sodium chloride, pH 7.1, with 15 mg/mL Bovine Serum Albumin (BSA) and 0.01% thimerosal.
Our affinity purified horseradish pero x idase is a 44kDa glycoprotein, with 4 lysine residues, for conjugation to a labeled molecule. It produces a colored, fluorimetric, or luminescent derivative of the labeled molecule, allowing it to be detected and quantified. HRP is ideal for secondary antibody conjugation because it is smaller, more stable, and less e x pensive than other popular alternatives. It also has a high turnover rate that allows the generation of strong signals in a relatively short time span. The activity of the HRP enzyme is inhibited by cyanides, azides, and sulfides.
Our affinity purified alkaline phosphatase is a large 140kDa protein that hydrolyzes phosphate groups from substrates, resulting in a colored, fluorimetric, or luminescent derivative.
Type : Secondary
Antigen : IgG
Conjugates :

• Alkaline Phosphatase (786-R43, 786-R44, 786-R45, 786-R46, 786-R47, 786-R49)
• Horseradish Pero x idase (786-R38, 786-R39, 786-R40, 786-R41, 786-R42, 786-R48)

Host :

• Goat (786-R38, 786-R39, 786-R40, 786-R41, 786-R43, 786-R44, 786-R45, 786-R46)
• Rabbit (786-R42, 786-R47, 786-R48, 786-R49)

Reactivity :

• Mouse (786-R38, 786-R43)
• Goat (786-R42, 786-R47)
• Rat (786-R40, 786-R45)
• Rabbit (786-R39, 786-R44)
• Human (786-R41, 786-R46, 786-R48, 786-R49)

Specifications:
ChIP No
ELISA Yes
Flow Cytometry Yes
Gel Shift No
Immunochemistry Yes
Immunofluorescence No
Immunoprecipitation No
Monoclonal/Polyclonal Polyclonal
Neutralization No
Western Blot Yes
Applications

• For antibody conjugation to labeled molecules
• Recommended Working Dilutions:

ELISA and Western blots 1:5, 000 to 1:100, 000
Immunohistochemistry 1:500 to 1:5, 000
AP labeled goat a-human IgG (0.6mg/mL), 1mL
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