G-Biosciences - BE-401 - Physical Properties of Proteins, For 6 groups of 4-5 or 24-30 students.

ITEM #: 14511293
Proteins are the building blocks of life and there are estimated to be almost 1 million different proteins in a normal animal cell. Each protein has very different and unique physical properties. The Physical Properties of Proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins.
Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilization.
This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-e x amine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be e x ploited for purification and characterization of proteins and apply their findings on a test sample of comple x tissue e x tract.
Supplied with components needed for hands-on e x perimentation for si x workstations of 4-5 students or 24-30 students. Supplied with Teacher’s Guide and separate Student’s Guides.
Features

• Physical properties of protein molecules in aqueous solution.

Applications

• Effect of temperature on protein solubility and denaturation.
• Effects of pH on protein solubility and protein isoelectric point.
• Role of salt on protein solubility and protein salting-out or precipitation.
• Interaction between protein and water molecules and hydrogen bonds
• Effects of organic solvent on dielectric constant and protein precipitation.
• The effects of detergents in altering the physical properties of proteins.

Physical Properties of Proteins, For 6 groups of 4-5 or 24-30 students.
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