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Genscript - L00689-30 - GENCRISPR SGRNA SCREENING KIT 30RXNS (Each)

  • MFR SKU: L00689-30
  • ITEM #: 15477436
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$202.70
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ITEM #: 15477436
Cas9 Nuclease NLS, S. pyogenes , is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif). The design of single guide RNA (sgRNA) is dependent on the target region close to the PAM site. Even if you pick a target sequence that fulfills all of the described requirements, sgRNA specificity and activity is unpredictable. Therefore, it is often recommended...
ITEM #: 15477436
Cas9 Nuclease NLS, S. pyogenes , is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif). The design of single guide RNA (sgRNA) is dependent on the target region close to the PAM site. Even if you pick a target sequence that fulfills all of the described requirements, sgRNA specificity and activity is unpredictable. Therefore, it is often recommended that multiple, different sgRNAs be designed to target a gene of interest. The GenCrispr sgRNA Screening Kit provides a simple, reliable, and rapid method for assessing sgRNA efficiency before cell transduction, allowing you to identify the highly effective CRISPR sgRNA.
Components Amount
30-reaction kit 100-reaction kit
GenCrispr Cas9 Nuclease 15 μL 50 μL
10X Reaction Buffer 100 μL 300 μL
Positive Control sgRNA 3 μg 10 μg
Positive Control Substrate 60 μL 200 μL
RNase-free water 1 mL 1 mL
GenCrispr sgRNA Screening Kit, 30reactions
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Genscript - L00689-30 - GENCRISPR SGRNA SCREENING KIT 30RXNS (Each)

  • Item #: 15477436
  • MFR SKU: L00689-30

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