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Genscript - Z03390-100 - GenCrispr NLS-Cas9-D10A Nickase, 100µg (4mg/ml)

  • MFR SKU: Z03390-100
  • ITEM #: 15500946
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ITEM #: 15500946
GenCrispr NLS-Cas9-D10A Nickase is a mutation form of Cas9 Nuclease. Cas9 nuclease is an RNA-guided endonuclease that can catalyze cleavage of double stranded DNA. This kind of targeted nuclease is a powerful tool for genome editing with high precision. Cas9 nuclease cleaves the double strand DNA generating two break sites based on its two active domains. NLS-Cas9-D10A Nickase is a mutation form of Cas9 Nuclease which makes one active domain deactivated, thus it can only cut one single strand DNA...
ITEM #: 15500946
GenCrispr NLS-Cas9-D10A Nickase is a mutation form of Cas9 Nuclease. Cas9 nuclease is an RNA-guided endonuclease that can catalyze cleavage of double stranded DNA. This kind of targeted nuclease is a powerful tool for genome editing with high precision. Cas9 nuclease cleaves the double strand DNA generating two break sites based on its two active domains. NLS-Cas9-D10A Nickase is a mutation form of Cas9 Nuclease which makes one active domain deactivated, thus it can only cut one single strand DNA that is complementary to the guide-RNA, producing one single strand cut. Combined with two different gRNA, NLS-Cas9-D10A Nickase produces two cut sites respectively and causes a double strand break. Compared with the wild type Cas9 nuclease, the two-gRNA guided cleavage can significantly reduce the off target effects.
Product Source: GenCripsr NLS-Cas9-D10A Nickase is highly purified mutant proteins e x pressed in an E. coli strain carrying a plasmid encoding the mutated Cas9 gene from Streptococcus pyogenes .
Key features:
  • DNA-free: no e x ternal DNA added to system.
  • High cleavage efficiency: NLS ensures the efficient entry of Cas9 protein into nuclei.
  • Low off target: Double gRNA-guided cleavage and transient e x pression of Cas9 nuclease.
  • Time-saving: no need for transcription and translation.

With this Cas9 Nuclease, you can:
  • Screening the highly efficient and specific targeting gRNAs using in vitro DNA cleavage.
  • In vivo gene editing combined with specific gRNA by electroporation or injection.

    • GenCrispr NLS-Cas9-D10A Nickase, 100µg (4mg/ml)
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    Genscript - Z03390-100 - GenCrispr NLS-Cas9-D10A Nickase, 100µg (4mg/ml)

    • Item #: 15500946
    • MFR SKU: Z03390-100
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