HiMedia - AT065A-20L - Dulbecco's Modified Eagle Medium (DMEM) (Modified for Autoclaving) with 1gm Glucose per liter and Sodium pyruvate, without L-Glutamine and Sodium bicarbonate, 20 L

ITEM #: 3522636
Dulbecco's Modified Eagle Medium is one of the most widely used modification of Eagle's medium. DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mg glucose per litre and was originally used to culture embryonic mouse cells.
C985E74, C985E75 and C985E76 is DMEM low glucose and is modified for autoclaving. Autoclavable medium offers a convenient alternative to membrane sterilized liquid medium and is modified to include heat stable components to ensure that product efficacy is maintained after autoclaving. L-glutamine is heat labile, hence has been omitted from the formulation. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
Storage and Shelf Life
1. All the powdered media and prepared liquid culture media should be stored at 2-8°C. Use before the e x piry date. In spite of above recommended storage condition, certain powdered medium may show some signs of deterioration /degradation in certain instances. This can be indicated by change in colour, change in appearance and presence of particulate matter and haziness after dissolution.
2. Preparation of concentrated medium is not recommended since free base amino acids and salt comple x es having low solubility may precipitate in concentrated medium.
3. pH and sodium bicarbonate concentration of the prepared medium are critical factors affecting cell growth. This is also influenced by amount of medium and volume of culture vessel used (surface to volume ratio). For e x ample, in large bottles, such as Rou x bottles pH tends to rise perceptibly as significant volume of carbon dio x ide is released. Therefore, optimal conditions of pH, sodium bicarbonate concentration, surface to volume ratio must be determined for each cell type. We recommend stringent monitoring of pH. If needed, pH can be adjusted by using sterile 1N HCl or 1N NaOH or by bubbling in carbon dio x ide.
4. If required, supplements can be added to the medium prior to or after filter sterilization observing sterility precautions. Shelf life of the medium will depend on the nature of supplement added to the medium.
Dulbecco's Modified Eagle Medium (DMEM) (Modified for Autoclaving) with 1gm Glucose per liter and Sodium pyruvate, without L-Glutamine and Sodium bicarbonate, 20 L
This listing is for Each