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IBI Scientific - IB47020 - Gel/PCR Fragment Extraction Kit - 100 Preps, EA

  • MFR SKU: IB47020
  • ITEM #: 5849075
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ITEM #: 5849075
Efficient gel e x traction and PCR clean-up
The Gel/PCR DNA Fragment E x traction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from an agarose gel, PCR, or any other enzymatic reaction. This method uses a chaotropic salt to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matri x of the spin column. The contaminants are washed with a wash buffer (containing Ethanol) and the purified DNA fragments...
ITEM #: 5849075
Efficient gel e x traction and PCR clean-up
The Gel/PCR DNA Fragment E x traction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from an agarose gel, PCR, or any other enzymatic reaction. This method uses a chaotropic salt to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matri x of the spin column. The contaminants are washed with a wash buffer (containing Ethanol) and the purified DNA fragments are eluted by a low salt based elution buffer or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mi x ture without phenol e x traction or alcohol precipitation. Typically, recoveries are 80-90% for gel e x traction and 90-95% for PCR clean-up. The entire procedure can be completed in 20 minutes, and the eluted DNA is ready to use in restriction digestion, ligation, PCR, and sequencing reactions.
Gel/PCR Fragment Extraction Kit-100 Preps
This listing is for Pack of 100
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IBI Scientific - IB47020 - Gel/PCR Fragment Extraction Kit - 100 Preps, EA

  • Item #: 5849075
  • MFR SKU: IB47020
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