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Intact Genomics - 3612 - T7 Endonuclease I - 1, 250 units

  • MFR SKU: 3612
  • ITEM #: 17841302
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ITEM #: 17841302
T7 Endonuclease I, a stable homodimer of identical 149 amino acid subunits (1) is the product of a recombinant gene in E. coli . Double-stranded breaks (DSBs) generated by CRISPR/TALEN at desired target sites can be PCR-amplified, and the PCR products can be denatured and re-annealed to form mismatched DNA. If the mismatched DNA length position is more than 1 bp, T7 endonuclease I can recognize and cleave it. It is useful for quantitatively estimating the nuclease-induced mutation frequency of gene...
ITEM #: 17841302
T7 Endonuclease I, a stable homodimer of identical 149 amino acid subunits (1) is the product of a recombinant gene in E. coli . Double-stranded breaks (DSBs) generated by CRISPR/TALEN at desired target sites can be PCR-amplified, and the PCR products can be denatured and re-annealed to form mismatched DNA. If the mismatched DNA length position is more than 1 bp, T7 endonuclease I can recognize and cleave it. It is useful for quantitatively estimating the nuclease-induced mutation frequency of gene edited cells.
Product Includes:
• T7 Endonuclease I
• 10 x T7 Endonuclease I reaction buffer
T7 Endonuclease I - 1, 250 units
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Intact Genomics - 3612 - T7 Endonuclease I - 1, 250 units

  • Item #: 17841302
  • MFR SKU: 3612

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