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Intact Genomics - 4117 - ig-Fusion Cloning Kit - 100 Reactions

  • MFR SKU: 4117
  • ITEM #: 17841311
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ITEM #: 17841311
Intact Genomics propriety ig-Fusion cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR product(s) to any linearized e x pression vector at any site. The PCR fragments can be generated by Intact Genomics high fidelity Pfu DNA polymerase or other high fidelity DNA polymerases, with primers having 15 to 18 bases of homology at their linear ends to where the product need to fuse. The linearized vector can be generated by PCR or restriction enzymes....
ITEM #: 17841311
Intact Genomics propriety ig-Fusion cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR product(s) to any linearized e x pression vector at any site. The PCR fragments can be generated by Intact Genomics high fidelity Pfu DNA polymerase or other high fidelity DNA polymerases, with primers having 15 to 18 bases of homology at their linear ends to where the product need to fuse. The linearized vector can be generated by PCR or restriction enzymes. The kit is so robust that multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single reaction step within short times (usually 10-30 min) with more than 95% cloning efficiency.
Applications:
Clone any insert at any site within any vector
Restriction enzyme and phosphatase free system
Joining multiple large fragments at once
Precise insertion at a desired orientation
Rapid and high efficiency with >95% positive clones
Product Includes:
5 x ig-Fusion enzyme premi x
2 x PCR premi x
High efficiency competent cells
ig-Fusion Cloning Kit - 100 Reactions
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Intact Genomics - 4117 - ig-Fusion Cloning Kit - 100 Reactions

  • Item #: 17841311
  • MFR SKU: 4117
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