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Integrated BioTherapeutics - 1401-002 - Recombinant Hla (tag free), 100ug

  • MFR SKU: 1401-002
  • ITEM #: 12981540
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$364.06
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ITEM #: 12981540
Description: Staphylococcus aureus alpha hemolysin (Hla), also known as alpha to x in (α-to x in), is e x pressed in E. coli as a recombinant protein without TAG. The theoretical molecular weight of the protein is appro x imately 33 kDa.
Size: 100 µg is supplied in phosphate buffered saline at a concentration of 0.857 mg/mL
Endoto x in: 8.029 Endoto x in units/mg
Relevance: Hla is produced by S. aureus and is a major cytoto x ic agent. It is e x pressed as a monomer...
ITEM #: 12981540
Description: Staphylococcus aureus alpha hemolysin (Hla), also known as alpha to x in (α-to x in), is e x pressed in E. coli as a recombinant protein without TAG. The theoretical molecular weight of the protein is appro x imately 33 kDa.
Size: 100 µg is supplied in phosphate buffered saline at a concentration of 0.857 mg/mL
Endoto x in: 8.029 Endoto x in units/mg
Relevance: Hla is produced by S. aureus and is a major cytoto x ic agent. It is e x pressed as a monomer that binds to the membrane of susceptible cells. Subunits then oligomerize to form heptameric rings with a central pore through which cellular contents leak. The mode of action of S. aureus Hla is likely by osmotic lysis
Recommended Dilutions:
ELISA: Assay-dependent dilution.
WB: Assay-dependent dilution; internal QC demonstrates detection of Hla protein using IBT’s mouse anti-Alpha To x in (6C12) monoclonal antibody (cat# 0210-005) in Western blotting.
Cytoto x icity assay: The protein is active in functional lysis assays with rabbit red blood cells. The protein can be also used in serology assay as coating antigen to detect antibodies against Hla.
Storage: -80°C
Intended for research use only, not for human, therapeutic, or diagnostic applications.
Recombinant Hla (tag free), 100ug
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Integrated BioTherapeutics - 1401-002 - Recombinant Hla (tag free), 100ug

  • Item #: 12981540
  • MFR SKU: 1401-002

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