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Merck - 03-0136-00 - SMC Mouse IL-4 Discovery Immunoassay Kit

  • MFR SKU: 03-0136-00
  • ITEM #: 11567755
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ITEM #: 11567755
The Single Molecule Counting (SMC™) Mouse IL-4 Discovery Immunoassay has unparalleled performance in quantifying IL-4 in mouse serum.

Merck - 03-0136-00 - SMC Mouse IL-4 Discovery Immunoassay Kit

  • Item #: 11567755
  • MFR SKU: 03-0136-00
Technical Specs
  • Catalogue Number 03-0136-00
  • Trade Name
  • SMC™
  • SMC™ Mouse IL-4 Discovery Immunoassay Kit
  • Overview The SMC™ Mouse IL-4 Discovery Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
  • Background Information Interleukin-4 (IL-4) is a cytokine that is a key regulator in humoral and adaptive immunity. IL-4 induces differentiation of naive helper T-cells (Th0 cells) to Th2 cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. Further, IL-4 controls the production of IgE, expands IL-4 producing T-cell subsets and stabilizes effector cell functions. IL-4 has great therapeutic potential due to its role in the development of allergic inflammatory responses. IL-4 also has shown to have promise in drug targeting for cancer.
  • Components
  • Assay Buffer
  • Mouse IL-4 Coating Antibody
  • Assay Plate
  • 10X PBS
  • Blocking Buffer
  • Standard Diluent
  • Mouse IL-4 Detection Antibody
  • Mouse IL-4 Standard
  • SMC™ Mouse IL-4 Immunoassay Kit Instructions
  • 10X Wash Buffer
  • Buffer C
  • Elution Buffer B
  • Precision, %
  • Low-end CV% Range: 0-20%
  • Low-end CV% Average: 4%
  • Quality Level MQ200
  • Application The Single Molecule Counting (SMC™) Mouse IL-4 Discovery Immunoassay has unparalleled performance in quantifying IL-4 in mouse serum.
  • Key Applications
  • Single Molecule Counting (SMC™)
  • Species Reactivity
  • Mouse
  • Analytes Available
  • IL-4
  • Accuracy
  • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
  • Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
  • Material Size 96 well assay
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