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Merck - 03-0137-00 - SMC Mouse TNF- Discovery Immunoassay Kit

  • MFR SKU: 03-0137-00
  • ITEM #: 11567866
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ITEM #: 11567866
The sensitivity of Single Molecule Counting (SMC™) technology gives the SMC™ Mouse TNF-α Discovery Immunoassay unparalleled performance in the quantification of TNF-α in mouse serum.

Merck - 03-0137-00 - SMC Mouse TNF- Discovery Immunoassay Kit

  • Item #: 11567866
  • MFR SKU: 03-0137-00
Technical Specs
  • Catalogue Number 03-0137-00
  • Trade Name
  • SMC™
  • SMC™ Mouse TNF-α Discovery Immunoassay Kit
  • Overview The SMC™ Mouse TNF-α Discovery Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
  • Background Information Tumor necrosis factor alpha (TNF-α) is a pro-inflammatory cytokine with cytotoxic activity that is primarily produced by macrophages. TNF-α is involved in systemic inflammation and is a member of the group of cytokines that stimulate the acute phase reaction. When administered to animals or humans it causes inflammation, fever, cardiovascular effects, hemorrhage, coagulation and acute phase responses similar to those seen during acute infections and shock states. By promoting the inflammatory response, TNF-α is associated with many autoimmune disorders such as rheumatoid arthritis, ankylosing spondylitis, Crohn’s disease, psoriasis and refractory asthma. TNF-α also plays a role in cancer and regulates apoptotic cell death, cellular proliferation, differentiation, tumorigenesis and viral replication.
  • Components
  • Assay Buffer
  • Mouse TNF-α Coating Antibody
  • Assay Plate
  • 10X PBS
  • Blocking Buffer
  • Standard Diluent
  • Mouse TNF-α Detection Antibody
  • Mouse TNF-α Standard
  • SMC™ Mouse TNF-α Immunoassay Kit Instructions
  • 10X Wash Buffer
  • Buffer C
  • Elution Buffer B
  • Precision, %
  • Low-end CV% Range: 0-16%
  • Low-end CV% Average: 6%
  • Quality Level MQ200
  • Application The sensitivity of Single Molecule Counting (SMC™) technology gives the SMC™ Mouse TNF-α Discovery Immunoassay unparalleled performance in the quantification of TNF-α in mouse serum.
  • Key Applications
  • Single Molecule Counting (SMC™)
  • Accuracy
  • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
  • Sensitivity
  • Lower Limit of Quantification: 0.49 pg/mL Upper Limit of Quantification: 500 pg/mL
  • Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
  • Material Size 96 well assay
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