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Merck - 03-250 - RIPAb+ Ago3 Antibody

  • MFR SKU: 03-250
  • ITEM #: 11572798
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$848.00
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ITEM #: 11572798
Ago3 (Eukaryotic translation initiation factor 2C 3) belongs to the argonaute family & contains a PAZ & a Piwi domain. Ago3 plays a role in the RNA interference (RNAi) pathway.

Merck - 03-250 - RIPAb+ Ago3 Antibody

  • Item #: 11572798
  • MFR SKU: 03-250
  • P-body.
  • Molecular Weight ~96 kDa
  • Quality Assurance RNA Binding Protein Immunoprecipitation: Representative lot data
  • RIP Lysate prepared from 293 cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal mouse IgG , or 5 µg Anti-Ago 3 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
  • Successful immunoprecipitation of Argonaute 3-associated RNA was verified by qPCR using RIP Primers IGF2, (Figure 1).
  • Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
  • Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
  • Storage Conditions Stable for 1 year at -20° C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20° C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
  • Note: Variability in freezer temperatures below -20° C may cause glycerol containing solutions to become frozen during storage.
  • Material Size 10 assays
  • Material Package 10 assays per set. Recommended use: ~5 µg of antibody per RIP (dependent upon biological context).

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