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Merck - 1000680500 - Oxiod Bolton Broth 500 g

  • MFR SKU: 1000680500
  • ITEM #: 3028954
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ITEM #: 3028954
For the selective enrichment of Campylobacter from foods
Formula: Mi x ture
Typical Composition (g/L): Peptone from meat 10.0; lactalbumin hydrolysate 5.0; yeast e x tract 5.0; sodium chloride 5.0; a-keto-glutaric acid 1.0; sodium pyruvate 0.5; sodium metabisulfate 0.5; sodium carbonate 0.6; hemin 0.01.
Oxiod Bolton Broth 500 g
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Merck - 1000680500 - Oxiod Bolton Broth 500 g

  • Item #: 3028954
  • MFR SKU: 1000680500
Technical Specs
  • Catalogue Number 100068
  • Overview GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.
  • HS Code 3821 00 00
  • Quality Level MQ200
  • pH value 7.2 - 7.6 (27.6 g/l, HO, 25 ° C) (after autoclaving)
  • Solubility 27.6 g/l
  • Hazard Pictogram(s)
  • Hazard Statement(s) H317: May cause an allergic skin reaction.
  • H318: Causes serious eye damage.
  • Precautionary Statement(s) P261: Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray.
  • P272: Contaminated work clothing should not be allowed out of the workplace.
  • P280: Wear protective gloves/ eye protection/ face protection.
  • P302 + P352: IF ON SKIN: Wash with plenty of water.
  • P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P333 + P313: If skin irritation or rash occurs: Get medical advice/ attention.
  • Signal Word Danger
  • Storage class 10 - 13 Other liquids and solids
  • WGK WGK 3 highly hazardous to water
  • Disposal 3
  • Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
  • Categories of danger irritant
  • Storage Store at +15° C to +25° C.
  • Appearance (color) dark red to black
  • pH-value (25° C) 7.2 - 7.6
  • Growth promotion test in accordance with the current version of DIN EN ISO 11133.
  • Mixed inoculum of Campylobacter, E.coli and P.mirabilis.
  • Additionaly monoculture of E.coli and P.mirabilis.
  • Afterwards confirmation on mCCDA resp. Tryptic soy agar.
  • Inoculum on reference medium (Campylobacter coli ATCC 43478 (WDCM 00004)) ≤ 100
  • Inoculum on reference medium (Campylobacter jejuni ATCC 33291 (WDCM 00005)) ≤ 100
  • Inoculum on reference medium (Campylobacter jejuni ATCC 29428 (WDCM 00156)) ≤ 100
  • Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)) ≥ 10000
  • Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)) ≥ 10000
  • Inoculum on reference medium (Proteus mirabilis ATCC 29906 (WDCM 00023)) ≥ 10000
  • Growth on mCCDA (Campylobacter coli ATCC 43478 (WDCM 00004)) > 10 CFU
  • Growth on mCCDA (Campylobacter jejuni ATCC 33291 (WDCM 00005)) > 10 CFU
  • Growth on mCCDA (Campylobacter jejuni ATCC 29428 (WDCM 00156)) > 10 CFU
  • Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)) no growth
  • Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)) no growth
  • Growth on Tryptic soy agar (Proteus mirabilis ATCC 29906 (WDCM 00023)) no growth
  • Colony color (Campylobacter coli ATCC 43478 (WDCM 00004)) greyish
  • Colony color (Campylobacter jejuni ATCC 33291 (WDCM 00005)) greyish
  • Colony color (Campylobacter jejuni ATCC 29428 (WDCM 00156)) greyish
  • Incubation: Bolton broth: 5 ± 1 hours at 37 ± 1 ° C, afterwards 44 ± 4 hours at 41.5 ± 1 ° C, Anaerocult C
  • Confirmation on mCCDA: at least 44 ± 4 hours at 41.5 ± 1 ° C, Anaerocult C
  • Confirmation on Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 ° C, aerobic
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