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Merck - 1092050500 - Tryptic Soy Broth Modified (With Novobiocin) 500G

  • MFR SKU: 1092050500
  • ITEM #: 3032277
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ITEM #: 3032277
Formula: Mi x ture
Synonym(s): Modified Tryptic Soy Broth (mTSB)
Description: Typical Composition (g/L): Peptone from casein 17.0; peptone from soymeal 3.0; sodium chloride 5.0; bile salts No. 3 1.5; D(+)glucose 2.5; di-potassium hydrogen phosphate 4.0; novobiocin 0.02.
Application: For the selective enrichment for enterohemorrhagic E. coli (EHEC) in foods, especially milk and dairy products.
Pkg. Type : Bottle, Poly
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Merck - 1092050500 - Tryptic Soy Broth Modified (With Novobiocin) 500G

  • Item #: 3032277
  • MFR SKU: 1092050500
Technical Specs
  • Catalogue Number 109205
  • HS Code 3821 00 00
  • Quality Level MQ200
  • pH value 7.1 - 7.5 (33 g/l, HO, 25 ° C) (after autoclaving)
  • Solubility 33 g/l
  • Storage class 10 - 13 Other liquids and solids
  • WGK WGK 3 highly hazardous to water
  • Disposal 3
  • Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
  • Storage Store at +15° C to +25° C.
  • Appearance (clearness) clear
  • Appearance (color) yellowish-brown
  • pH-value (25° C) 7.2 - 7.6
  • Growth promotion test in accordance with the current version of DIN EN ISO 11133.
  • Mixed inoculum of E.coli and S.aureus
  • Additionaly monoculture of S.aureus
  • Inoculum on reference medium (Escherichia coli ATCC 35150 (O157)) ≤ 100
  • Inoculum on reference medium (Escherichia coli ATCC 700728(WDCM 00014)) ≤ 100
  • Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)) ≤ 100
  • Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)) ≥ 10000
  • Growth (Escherichia coli ATCC 35150 (O157)) > 10 yellowish-brown colonies on CT-SMAC agar
  • Growth (Escherichia coli ATCC 700728 (WDCM 00014)) > 10 yellowish-brown colonies on CT-SMAC agar
  • Growth Escherichia coli ATCC 25922 (WDCM 00013) > 10 pink colonies on CT-SMAC agar
  • Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)) total inhibition on TSA
  • Incubation: mTSB broth: 18 - 24 hours at 41, 5 ° C, aerobic
  • Confirmation on CT-SMAC Agar resp. TSA: 24 ± 3 hours at 37 ± 1 ° C aerobic
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