Merck - 11696505001 - Agarose Gel DNA Extraction Kit, kit of for up to 100 reactions

ITEM #: 14260373
UNSPSC Code: 12352200
RIDADR: UN 1502 5.1 / PGII
Features and Benefits:
• Quick and simple.
E x tract high yields of DNA in only 45 minutes, with few hands-on steps.
• Combine with different agaroses and buffer systems.
Low melting point agarose is not required.
• Purify efficiently.
Highly specific binding of DNA allows easy removal of impurities.
• Isolate large DNA fragments without shearing.
Silica particles are uniform in size and have smooth surfaces, ensuring recovery of intact DNA fragments up to 100 kb.
• Isolate oligonucleotides >=20 bp.
Narrow size distribution of the silica particles and absence of fines ensure high binding capacity of the matri x .
• Avoid enzymatic inhibition.
No fine particles are observed in the suspension. The recovered product will not inhibit subsequent enzymatic reactions.
Application:
The Agarose DNA E x traction Kit efficiently isolates both small and large DNA fragments from standard or low melting point agarose. Recovered DNA fragments are suitable for:
• Ligation and transformation
• Enzymatic restriction
• Random primed or nick translation labeling methods
• Sequencing
• PCR/long PCR
• Cloning
• Concentrate dilute nucleic acid solutions
General description:
For the elution of DNA fragments from agarose gels.
The Agarose Gel DNA E x traction Kit provides convenient isolation of high-quality DNA in large amounts.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
DNA Molecular Weight Marker II is separated in a 0.8% agarose gel and the 546-, 2, 322-, and 6, 557 bp fragments are isolated with the kit components. The e x pected amount of DNA is recovered for each fragment. Depending on parameters such as fragment length, preparation, and purity of the applied DNA, up to 80% recovery is achieved. DNA fragments are digested with restriction enzymes. No inhibition of DNA digestion is observed.
Components:
• Silica Matri x , for 100 standard reactions
• Solubilization Buffer
• Nucleic Acid Binding Buffer
• Wash Buffer
Preparation Note:
A solubilization buffer containing a chaotropic salt (sodium perchlorate) dissolves an agarose gel slice that contains a DNA fragment. In the presence of the chaotropic salt, the DNA fragment binds selectively to silica matri x . The DNA remains bound while a series of rapid wash-and-spin steps remove contaminating small molecules. Finally, low-salt elution removes the DNA from the silica matri x . The process does not require DNA precipitation, organic solvent e x tractions, or e x tensive handling of the DNA.
Agarose Gel DNA Extraction Kit, kit of for up to 100 reactions
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