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Merck - 14-1057 - Human native nucleosomes

  • MFR SKU: 14-1057
  • ITEM #: 11573322
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ITEM #: 11573322
Human native nucleosomes purified from HeLa cell nuclei, digested with micrococcal nuclease & purified by glycerol gradient & ion exchange chromatography.

Merck - 14-1057 - Human native nucleosomes

  • Item #: 11573322
  • MFR SKU: 14-1057
  • Catalogue Number 14-1057
  • Brand Family Upstate
  • Trade Name
  • Upstate
  • Human native nucleosomes
  • Overview Human native nucleosomes purified from HeLa cell nuclei, digested with micrococcal nuclease, and purified by glycerol gradient and ion exchange chromatography.
  • Background Information Nucleosomes are the basic units of chromatin, and are comprised of approximately 147 bp of DNA spooled around a central octamer of core histones, H2A, H2B, H3, and H4. The arrangement of nucleosomes in chromatin can be altered by post-translational modifications (PTM) occurring on the C- and N-terminal tails of the core histones. Histone modifications can either provide or hinder access of key transcriptional complexes to DNA sequences and can regulate the expression of genes. These effects can be deciphered by applications such as chromatin immunoprecipiation (ChIP), which requires high quality chromatin, and essential tools such as histone proteins and appropriate enzymes, to assemble chromatin in vitro.
  • Presentation 20 μg of mononucleosomes in buffer containing 20 mM Tris-Cl pH 7.9, 1 mM EDTA, 0.5 mM PMSF, 1 mM DTT, and 50% glycerol.
  • Quality Level MQ100
  • Application Human native nucleosomes purified from HeLa cell nuclei, digested with micrococcal nuclease & purified by glycerol gradient & ion exchange chromatography.
  • Key Applications
  • Activity Assay
  • Application Notes Suitable for in vitro chromatin assembly and enzyme activity assays
  • Concentration 0.2 µg/µ L
  • Purity >98% purity by SDS PAGE
  • Source Human
  • Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
  • Storage Conditions Stable at -80º C for 1 year from date of receipt.
  • Material Size 20 µg

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