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Merck - 152224-10ML - AEC 10ML (Each)

  • MFR SKU: 152224-10ML
  • ITEM #: 6310678
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ITEM #: 6310678
AEC 10Ml^ AEC, 3-Amino-9-ethylcarbazole, 3AC, Form Yellowish-brown to reddish solution, Chemical formula: C14H14N2, Cas number: 132-32-1, Applications : Immunoblotting (Western Blotting), Immunohistochemistry
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Merck - 152224-10ML - AEC 10ML (Each)

  • Item #: 6310678
  • MFR SKU: 152224-10ML
  • Overview Precipitable peroxidase substrate for immunoblotting and immunohistochemical staining techniques. Produces a red end product that is soluble in alcohol. When used in immunohistochemistry, aqueous mounting media, and counterstains must be used. Directions included for use in each application.
  • Catalogue Number 152224
  • Brand Family Calbiochem®
  • Synonyms 3-Amino-9-ethylcarbazole, 3AC
  • References Okano, K., et al. 1992. Rinsho Byori 40, 93.
  • Krenacs, T., et al. 1990. Histochem. J. 22, 530.
  • CAS number 132-32-1
  • Form Yellowish-brown to reddish solution
  • Hill Formula CHN
  • Chemical formula CHN
  • Preservative None
  • Quality Level MQ100
  • Key Applications Immunoblotting (Western Blotting)
  • Immunohistochemistry
  • Application Notes Immunoblotting (see comments)
  • Immunohistochemistry (see comments)
  • Application Comments Stable at 4° C and 18-26° C. Functionality determined by performing an immunohistologic stain using the final product.
  • Suggested procedure for use of AEC in immunohistochemistry and immunoblotting: 1. Complete all required incubations with antibodies and HRP-labeled probes, and all necessary wash steps.
  • 2.Prepare 1X AEC Substrate Buffer: Add 9 parts distilled water to 1 part 10X AEC Substrate Buffer, Cat. No. 152226 (0.5 M acetate buffer containing 88 mM H 2 O 2 ). Mix well. The final pH of this solution will be ~5.0.
  • 3. Prepare 1X AEC Substrate Solution: Add 1 part 50X AEC Concentrate to 50 parts 1X AEC Substrate Buffer (prepared in Step 2). Mix well.
  • 4. Following the final wash, completely cover tissue sections with 1X AEC Substrate Solution (prepared in Step 3) and incubate 10-30 min at room temperature. The same conditions apply for immunoblotting. Note: Variables associated with assay conditions will dictate the proper reaction times.
  • 5. After reaction is complete, wash tissue sections or blotting membranes thoroughly in distilled water.
  • 6. If tissue sections require a counterstain, Mayer's Hematoxylin followed by blueing in alkaline tap water or a 0.5% aqueous methyl green solution is recommended.
  • 7. Mount tissue sections in a water-based mounting medium. Air-dry blots and store protected from light.
  • RTECS FE3590000
  • R Phrase R: 25
  • Toxic if swallowed.
  • S Phrase S: 45
  • In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
  • Ship Code Ambient Temperature Only
  • Toxicity Toxic
  • Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
  • Storage +2° C to +8° C
  • Protect from Light Protect from light
  • Do not freeze Ok to freeze
  • Special Instructions Discard if a red or brown precipitate is evident.

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