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Merck - 152226-50ML - AEC SUBSTRATE BUFFER 50ML (Each)

  • MFR SKU: 152226-50ML
  • ITEM #: 5853825
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ITEM #: 5853825
AEC Substrate Buffer 50Ml^ AEC Substrate Buffer, Form Liquid, Formulation In 500 mM acetate buffer, 88 mM H2O2, proprietary stabilizers.Applications: Immunoblotting (Western Blotting), Immunohistochemistry
This listing is for Each

Merck - 152226-50ML - AEC SUBSTRATE BUFFER 50ML (Each)

  • Item #: 5853825
  • MFR SKU: 152226-50ML
Technical Specs
  • Overview Peroxidase substrate buffer supplied as a 10X concentrate (pH 5.0 when diluted). A diluent for Cat. No. 152224 .
  • Catalogue Number 152226
  • Brand Family Calbiochem®
  • Form Liquid
  • Formulation In 500 mM acetate buffer, 88 mM HO, proprietary stabilizers.
  • Preservative None
  • Quality Level MQ100
  • Key Applications Immunoblotting (Western Blotting)
  • Immunohistochemistry
  • Application Notes Immunoblotting (see comments)
  • Immunohistochemistry (see comments)
  • Application Comments Stable at 4° C, 18-26° C, and 37° C. When diluted the final pH is ~5.04. Functionality tested by performing immunohistologic stain using the final product.
  • Suggested procedure for use of AEC in immunohistochemistry and immunoblotting: 1. Complete all required incubations with antibodies and HRP-labeled probes, and all necessary wash steps.
  • 2. Prepare 1X AEC Substrate Buffer: Add 9 parts distilled water to 1 part 10X AEC Substrate Buffer, Cat. No. 152226 (0.5 M acetate buffer containing 88 mM H 2 O 2 ). Mix well. The final pH of this solution will be ~5.0.
  • 3. Prepare 1X AEC Substrate Solution: Add 1 part 50X AEC Concentrate (Cat. No. 152224) to 50 parts 1X AEC Substrate Buffer (prepared in Step 2). Mix well.
  • 4. Following the final wash, completely cover tissue sections with 1X AEC Substrate Solution (prepared in Step 3) and incubate 10-30 min at room temperature. The same conditions apply for immunoblotting. Note: Variables associated with assay conditions will dictate the proper reaction times.
  • 5. After reaction is complete, wash tissue sections or blotting membranes thoroughly in distilled water.
  • 6. If tissue sections require a counterstain, Mayer's Hematoxylin followed by blueing in alkaline tap water or a 0.5% aqueous methyl green solution is recommended.
  • 7. Mount tissue sections in a water-based mounting medium. Air-dry blots and store protected from light.
  • Ship Code Ambient Temperature Only
  • Toxicity Standard Handling
  • Storage +2° C to +8° C
  • Do not freeze Ok to freeze
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