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Merck - 208712-1MG - CALPAIN I PORCINE ERY 1 MG (Each)

  • MFR SKU: 208712-1MG
  • ITEM #: 5015639
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ITEM #: 5015639
Calpain I, Porcine Erythrocytes^ (m-Calpain). Liquid. In 20 mM imidazole-HCl, 5 mM b-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8. DO NOT FREEZE. Ca2+-dependent cysteine proteinase with low Ca2+ requirement (half-maximal activation = 2 mM).
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Merck - 208712-1MG - CALPAIN I PORCINE ERY 1 MG (Each)

  • Item #: 5015639
  • MFR SKU: 208712-1MG
  • Overview Native calpain-1 from porcine erythrocytes. Ca 2+ -dependent heterodimeric cysteine proteinase with low Ca 2+ requirement (EC 50 = 2 µ M). Participates in the ATP release reaction of platelets stimulated with thrombin.
  • Catalogue Number 208712
  • Brand Family Calbiochem®
  • Synonyms μ-Calpain
  • References Vanderklish, P.W., and Bahr, B.A. 2000. Int. J. Exp. Pathol. 81, 323.
  • Sorimachi, H., et al. 1997. Biochem. J. 328, 721.
  • Kampfl, A., et al. 1997. J. Neurotrauma 14, 121.
  • Johnson, G.V.W., and Gutmann, R.P. 1997. BioEssays 19, 1011.
  • Bartus, R.T., et al. 1995. Neurol. Res. 17, 249.
  • Wang, K.K.W., and Yuen, P.-W. 1994. Trends Pharmacol. Sci. 15, 412.
  • Saito, K., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 2628.
  • Goll, D.E., et al. 1992. BioEssays 14, 549.
  • Ishii, H., et al. 1992. Biochim. Biophys. Acta 1175, 37.
  • Melloni, E., and Pontremoli, S. 1989. Trends Neurosci. 12, 438.
  • Ross, E., and Schatz, G. 1973. Anal. Biochem. 54, 304.
  • Unit of Definition One unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 min at 25° C using the Calpain Activity Assay Kit, Fluorogenic (Cat. No. QIA120). Note: 1 caseinolytic unit = 9 fluorogenic units
  • EC number 3.4.22.17
  • Form Liquid
  • Formulation In 20 mM imidazole-HCl, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8.
  • PI 5.3
  • Quality Level MQ100
  • Specific Activity ≥ 1000 units/mg protein
  • Concentration Label Please refer to vial label for lot-specific concentration
  • R Phrase R: 36/37/38-42/43
  • Irritating to eyes, respiratory system and skin.
  • May cause sensitization by inhalation and skin contact.
  • S Phrase S: 26-36
  • In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
  • Wear suitable protective clothing.
  • Ship Code Dry Ice Only
  • Toxicity Harmful
  • Storage ≤ -70° C
  • Avoid freeze/thaw Avoid freeze/thaw
  • Do not freeze Ok to freeze
  • Special Instructions Following initial thaw, aliquot and freeze (-70° C). Short-term storage of aliquots at 4° C or on ice is not recommended.

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