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Merck - 208773-1MG - Calpain Substrate IV, Cell-Permeable, Fluorogenic | Calbiochem | 1MG

  • MFR SKU: 208773-1MG
  • ITEM #: 11574754
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ITEM #: 11574754
The non-permeant fluorogenic Calpain Substrate III is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage.

Merck - 208773-1MG - Calpain Substrate IV, Cell-Permeable, Fluorogenic | Calbiochem | 1MG

  • Item #: 11574754
  • MFR SKU: 208773-1MG
  • Overview The non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771 ) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity ( k cat = 0.2 s -1 , k cat / K M = 5000 M -1 s -1 ) when compared to the parental peptide ( k cat = 0.17 s -1 , k cat / K M = 680 M -1 s -1 ). Its suitability in detecting cellular Ca 2+ -dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca 2+ addition.
  • Catalogue Number 208773
  • Brand Family Calbiochem®
  • Synonyms (DABCYL)-TPLK~SPPPSPRE(EDANS)-RRRRRRR-NH
  • References Banoczi, Z., et al. 2008. Bioconjug. Chem. 19, 1375.
  • Form Lyophilized solid
  • Formulation Supplied as a trifluoroacetate salt
  • Hill Formula CHNOS
  • Chemical formula CHNOS
  • Hygroscopic Hygroscopic
  • Quality Level MQ100
  • Purity ≥ 95% by HPLC
  • Emission max.
  • Excitation max.
  • Peptide Sequence (DABCYL)-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-Glu(EDANS)-Arg-Arg-Arg-Arg-Arg-Arg-Arg-NH
  • Ship Code Ambient Temperature Only
  • Toxicity Standard Handling
  • Storage -20° C
  • Protect from Light Protect from light
  • Hygroscopic Hygroscopic
  • Do not freeze Ok to freeze
  • Special Instructions Following reconstitution, aliquot and freeze (-20° C). Stock solutions are stable for up to 3 months at -20° C.
  • Packaged under inert gas Packaged under inert gas

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