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Merck - 216254-1MG - BCECF/AM | CAS 117464-70-7 | Calbiochem | 1MG

  • MFR SKU: 216254-1MG
  • ITEM #: 3029273
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ITEM #: 3029273
BCECF/AM Bastadin^ 1mg white to off-white solid. Protect from light. Packaged under inert gas. Purity>=90% by HPLC. Soluble in DMF or DMSO. CAS 117464-70-7. May cause irritation. Cell-permeable ester deriviatives of BCECF that are hydrolyzed to yield intracellularly-trapped indicator BCECF.
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Merck - 216254-1MG - BCECF/AM | CAS 117464-70-7 | Calbiochem | 1MG

  • Item #: 3029273
  • MFR SKU: 216254-1MG
  • Overview A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules.
  • Catalogue Number 216254
  • Brand Family Calbiochem®
  • Synonyms 2, 7- bis -(Carboxyethyl)-5(6)-carboxyfluorescein Acetoxymethyl Ester
  • References Futsaetner, C.M., et al. 1993. Can. J. Microbiol. 39, 180.
  • Hayashi, H., et al. 1992. Am. J. Physiol . 262, C628.
  • Sasaki, S., et al. 1992. Biochim. Biophys. Acta 1137, 45.
  • Miyata, H., et al. 1989. Biochem. Biophys. Res. Commun. 163, 500.
  • Weiner, I.D., and Hamm, L.L. 1989. Am. J. Physiol. 256, F957.
  • CAS number 117464-70-7
  • ATP Competitive N
  • Form White to off-white solid
  • Reversible N
  • Quality Level MQ100
  • Primary Target Monitor intracellular pH changes
  • Purity ≥ 85% by HPLC (mixture of isomers)
  • Cell permeable Y
  • Emission max.
  • Excitation max.
  • R Phrase R: 36/37/38
  • Irritating to eyes, respiratory system and skin.
  • S Phrase S: 36-22-26
  • Wear suitable protective clothing.
  • Do not breathe dust.
  • In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
  • Ship Code Ambient Temperature Only
  • Toxicity Irritant
  • Storage -20° C
  • Protect from Light Protect from light
  • Do not freeze Ok to freeze
  • Special Instructions Following reconstitution, aliquot and freeze (-20° C). Stock solutions are stable for up to 1 week at -20° C.
  • Packaged under inert gas Packaged under inert gas

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