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Merck - 219360-1MG - Cathepsin D/E Substrate, Fluorogenic | Calbiochem | 1MG

  • MFR SKU: 219360-1MG
  • ITEM #: 6281492
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ITEM #: 6281492
Cathepsin D/E Substrate, Fluorogenic, is an internally quenched fluorogenic 11-mer peptide that acts as a selective substrate for cathepsins D and E.
This listing is for 1 mg Plastic ampoule

Merck - 219360-1MG - Cathepsin D/E Substrate, Fluorogenic | Calbiochem | 1MG

  • Item #: 6281492
  • MFR SKU: 219360-1MG
  • Overview An internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E (k cat /K m = 15.6 µ M -1 s -1 and 10.9 µ M -1 s -1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively), but not cathepsins B, H, or L . Cleavage occurs between Phe-Phe residues and results in enhanced fluorescence. A useful tool for inhibitors screening or for the determination of cathepsins D and E activities in crude extracts of various tissues and cell lysates.
  • Catalogue Number 219360
  • Brand Family Calbiochem®
  • Synonyms MOCAc-GKPILF~FRLK(Dnp)- D -R-NH
  • References Yasuda, Y., et al. 1999. J. Biochem. 126, 260.
  • Yasuda, Y., et al. 1999. J. Biochem. 125, 1137.
  • ATP Competitive N
  • Form Yellow lyophilized solid
  • Formulation Supplied as a trifluoroacetate salt.
  • Hill Formula CHNO
  • Chemical formula CHNO
  • Hygroscopic Hygroscopic
  • Reversible N
  • Quality Level MQ100
  • Application Cathepsin D/E Substrate, Fluorogenic, is an internally quenched fluorogenic 11-mer peptide that acts as a selective substrate for cathepsins D and E.
  • Primary Target Substrate for cathepsins D and E 50 $anchor_PDP_OverviewTab_Product_Biological_Info_Primary Target IC 50 " value=" Biological Information"
  • br> Primary Target IC<sub> 50</sub> kcat/Km = 15.6 µ M¹ sec¹ and 10.9 µ M¹ sec¹ for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively
  • Purity ≥ 97% by HPLC
  • Cell permeable N
  • Emission max.
  • Excitation max.
  • Peptide Sequence MOCAc-Gly-Lys-Pro-Ile-Leu-Phe~Phe-Arg-Leu-Lys(Dnp)- D -Arg-NH
  • MOCAc = (7-Methoxycoumarin-4-yl)acetyl
  • Ship Code Ambient Temperature Only
  • Toxicity Standard Handling
  • Storage +2° C to +8° C
  • Protect from Light Protect from light
  • Hygroscopic Hygroscopic
  • Do not freeze Ok to freeze
  • Special Instructions Following reconstitution aliquot and freeze (-20° C). Stock solutions are stable for up to 3 months at -20° C.
  • Packaged under inert gas Packaged under inert gas

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