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Merck - 251520-1MG - DAF-FM DA | CAS 254109-22-3 | Calbiochem | 1 MG

  • MFR SKU: 251520-1MG
  • ITEM #: 4149991
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ITEM #: 4149991
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.
This listing is for 1 mg Glass bottle

Merck - 251520-1MG - DAF-FM DA | CAS 254109-22-3 | Calbiochem | 1 MG

  • Item #: 4149991
  • MFR SKU: 251520-1MG
  • Overview A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515 ) by the action of esterases. Reacts with NO, in the presence of O 2 , resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.
  • Catalogue Number 251520
  • Brand Family Calbiochem®
  • Synonyms 3-Amino, 4-aminomethyl-2, 7-difluorofluorescein Diacetate
  • References Kojima, H., et al. 2001. J. Neurochem. 76, 1404.
  • Park. J.M., et al. 2001. Br. J. Pharmacol. 132, 1876.
  • Itoh, Y., et al. 2000. Anal. Biochem. 287, 203.
  • Kojima, H., et al. 1999. Angew Chem. Int. Ed. 38, 3209.
  • CAS number 254109-22-3
  • ATP Competitive N
  • Declaration Manufactured by Daiichi Pure Chemicals Co., Ltd
  • Form Pale yellow solution
  • Formulation A 5 mM solution in DMSO.
  • Hill Formula CHFNO
  • Chemical formula CHFNO
  • Reversible N
  • Structure formula Image
  • Quality Level MQ100
  • Primary Target Nitric oxide (NO) fluorescent indicator
  • Purity ≥ 98% by HPLC
  • Cell permeable Y
  • Emission max.
  • Excitation max.
  • R Phrase R: 36/38
  • Irritating to eyes and skin.
  • S Phrase S: 26
  • In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
  • Ship Code Ambient Temperature Only
  • Toxicity Irritant
  • Storage -20° C
  • Protect from Light Protect from light
  • Do not freeze Ok to freeze
  • Special Instructions Following initial thaw, aliquot and freeze at (-20° C). Use immediately after dilution; discard any unused portion.

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