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Merck - 281751-10ML - DAB, Tetrahydrochloride, 50X Concentrate

  • MFR SKU: 281751-10ML
  • ITEM #: 3441805
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ITEM #: 3441805
DAB 10Ml^ Tetrahydrochloride, 50X Concentrate 3, 3'-Diaminobenzidine Form Dark brown solution Cas number 7411-49-6 Storage +2C to +8C.Produces a brown alcohol-insoluble end product. Also useful as a stain for myelin in glutaraldehyde-fixed sections. Supplied as a 50X concentrate.
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Merck - 281751-10ML - DAB, Tetrahydrochloride, 50X Concentrate

  • Item #: 3441805
  • MFR SKU: 281751-10ML
Technical Specs
  • Overview Produces a brown alcohol-insoluble end product. Also useful as a stain for myelin in glutaraldehyde-fixed sections. Supplied as a 50X concentrate.
  • Catalogue Number 281751
  • Brand Family Calbiochem®
  • Synonyms 3, 3-Diaminobenzidine
  • References Krueger, S.K., et al. 1999. Biotech. Histochem. 74, 105.
  • Larrson, L.I. 1988. Immunocytochemistry: Theory and Practice, CRC Press, Inc., Boca Raton, FL.
  • Gallyas, F., et al. 1982. J. Histochem. Cytochem. 30, 183.
  • Hsu, S.M. and Soban, E. 1982. J. Histochem. Cytochem. 30, 1079.
  • Lewis, P.R. and Knight, D.P. 1977. Staining Methods for Sectioned Material, In Practical Methods in Electron Microscopy, A.M. Glauert, ed. North-Holland, Amsterdam.
  • Graham, R.C., Jr. and Karnovsky, M.J. 1966. J. Histochem. Cytochem. 14, 291.
  • CAS number 7411-49-6
  • Form Brown to dark brown solution
  • Formulation Supplied as a 50X concentrate.
  • Preservative None
  • Quality Level MQ100
  • Application Notes Immunoblotting (see comments)
  • Immunohistochemistry (see comments)
  • Application Comments Stable at 4° C and 18-26° C.
  • Suggested Procedure for Immunohistochemical Staining Using DAB: 1. Complete all required incubations with antibodies and HRP-labeled probes, and all necessary wash steps.
  • 2. Prepare 1X DAB Substrate Buffer: Add 9 parts distilled water to 1 part 10X DAB Substrate Buffer (Cat. No. 281753). Mix well. [Note: 1 M Tris-HCl, pH 7.6 may be substituted for Cat. No. 281753, such that the final (1X) concentration is 100 mM Tris-HCl, pH 7.6.
  • 3. Prepare 1X DAB Substrate Solution: Add 1 part 50X DAB Concentrate and 1 part 0.5% H 2 O 2 to 50 parts 1X DAB Substrate Buffer (prepared in Step 2). Mix well and protect from light.
  • 4. Following the final wash, completely cover tissue sections with the 1X DAB Substrate Solution (prepared in Step 3) and incubate 5-15 min at room temperature. Note: Variables associated with assay conditions will dictate the proper reaction time.
  • 5. After reaction is complete, wash tissue sections thoroughly in distilled water.
  • 6. Counterstain with hematoxylin if desired.
  • 7. Dehydrate in graded alcohols, xylene, or xylene substitutes.
  • 8. Mount tissue sections using xylene-based mounting media.
  • Suggested Procedure for Immunoblot Staining with DAB: 1. Complete all required incubations with antibodies and HRP-labeled probes, and all necessary wash steps.
  • 2. Prepare 1X DAB Substrate Buffer: Add 9 parts distilled water to 1 part 10X DAB Substrate Buffer (Cat. No. 281753). Mix well. [Note: 1 M Tris-HCl, pH 7.6 may be substituted for Cat. No. 281753, such that the final (1X) concentration is 100 mM Tris-HCl, pH 7.6.]
  • 3. Prepare 1X DAB Substrate Solution: Add 1 part 50X DAB Concentrate and 1 part 0.5% H 2 O 2 to 50 parts 1X DAB Substrate Buffer (prepared in Step 2). Mix well and protect from light.
  • 4. Following the final wash, completely cover membranes with 1X DAB Substrate Solution (prepared in Step 3). Incubate 5-30 min at room temperature.
  • Note: Variables associated with assay conditions will dictate the proper reaction time.
  • 5. After reaction is complete, wash membranes thoroughly in distilled water.
  • 6. Air-dry membranes and store protected from light.
  • RTECS DV8753000
  • Ship Code Ambient Temperature Only
  • Toxicity Toxic
  • Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
  • Storage +2° C to +8° C
  • Do not freeze Ok to freeze
  • Special Instructions Discard if a precipitate forms or if reagent is purple (the purple material is a DAB oxidation product that binds avidly to heme-containing proteins).
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