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Merck - 309800-25MG - Dihydroethidium | CAS 38483-26-0 | Calbiochem | 25MG

  • MFR SKU: 309800-25MG
  • ITEM #: 6280868
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ITEM #: 6280868
Dihydroethidium^ 25mg. DHE. 2, 7-Diamino-10-ethyl-9-phenyl-9, 10-dihydrophenanthridine. Violet solid. Cell-permeable, chemically-reduced ethidium derivative. For detection of reactive oxygen species (ROS). Purity>=95% by TLC. RTECS SF7935000, CAS 38483-26-0.
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Merck - 309800-25MG - Dihydroethidium | CAS 38483-26-0 | Calbiochem | 25MG

  • Item #: 6280868
  • MFR SKU: 309800-25MG
  • Overview A cell-permeable, chemically-reduced ethidium derivative. Commonly used to analyze respiratory burst in phagocytes. Useful fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, whereas after oxidation of this probe to ethidium, it intercalates cell’s DNA, staining the nucleus with a bright red fluorescence.
  • Catalogue Number 309800
  • Brand Family Calbiochem®
  • Synonyms 2, 7-Diamino-10-ethyl-9-phenyl-9, 10-dihydrophenanthridine, DHE
  • References Haugen, T.S., et al. 1999. Eur. Respir. J. 14, 1100.
  • Mancini, M., et al. 1997. J. Cell Biol. 138, 449.
  • Carter, W.O., et al. 1994. J. Leukoc. Biol. 55, 253.
  • Cinco, M., et al. 1994. FEMS Microbiol. Lett . 122, 187.
  • Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117.
  • Perticarari, S., et al. 1991. Cytometry 12, 687.
  • Bucana, C.D., et al. 1990. Exp. Cell Res. 190, 69.
  • Rothe, G., and Valet, G. 1990. J. Leukoc. Biol. 47, 440.
  • CAS number 38483-26-0
  • ATP Competitive N
  • Form Pink solid
  • Hill Formula CHN
  • Chemical formula CHN
  • Reversible N
  • Structure formula Image
  • Quality Level MQ100
  • Primary Target Useful fluorogenic probe for the detection of reactive oxygen species (ROS)
  • Purity ≥ 95% by HPLC
  • Cell permeable Y
  • RTECS SF7935000
  • R Phrase R: 36/37/38
  • Irritating to eyes, respiratory system and skin.
  • S Phrase S: 26-36
  • In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
  • Wear suitable protective clothing.
  • Ship Code Ambient Temperature Only
  • Toxicity Irritant
  • Storage -20° C
  • Protect from Light Protect from light
  • Do not freeze Ok to freeze
  • Special Instructions Following reconstitution, aliquot, flush with an inert gas, and freeze (-20° C). Stock solutions are stable for up to 6 months at -20° C.
  • Packaged under inert gas Packaged under inert gas

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