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Merck - 324715-5UG - Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

MFR SKU: 324715-5UG

ITEM #: 7010048

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$477.50

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ITEM #: 7010048
Endoproteinase Lys-C, Excision Grade, Lysobacter enzymogenes^ Solid lyophilized from 50 mM tricine, 10 mM EDTA, pH 8.0. HYGROSCOPIC. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins.
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Merck - 324715-5UG - Endoproteinase Lys-C, Sequencing Grade, <i>Lysobacter enzymogenes</i>

Item #: 7010048

MFR SKU: 324715-5UG

Technical Specs

Overview Native endoproteinase Lys-C from Lysobacter enzymogenes . Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
Catalogue Number 324715
Brand Family Calbiochem®
References Jekel, P.A., et al. 1983. Anal. Biochem. 134, 347.
CAS number 72561-05-8
Unit of Definition One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-<i>p</i> NA per min at 25° C, pH 7.7.
EC number 3.4.21.50
Form Lyophilized
Formulation Lyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.
Hygroscopic Hygroscopic
Quality Level MQ100
Purity ≥ 90% by SDS-PAGE

Specific Activity ≥ 200 units/mg protein
R Phrase R: 20-36/37/38-42/43
Harmful by inhalation.
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
S Phrase S: 22-26-36
Do not breathe dust.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +2° C to +8° C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special Instructions Following reconstitution, aliquot and freeze (-20° C) for long term storage or refrigerate (4° C) for short-term storage. Stock solutions are stable for up to 2 days at 4° C or for up to 1 month at -20° C.

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