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Merck - 33-039 - PI 3-Kinase Class II HTRF Assay

  • MFR SKU: 33-039
  • ITEM #: 11575025
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ITEM #: 11575025
Five-plate kit (1920 wells) of reagents for performing PI 3-Kinase (Class II) 4-step HTRF assays. Suitable for the following Kinases: PI3KC2α, PI3KC2β & PI3KC2γ.

Merck - 33-039 - PI 3-Kinase Class II HTRF Assay

  • Item #: 11575025
  • MFR SKU: 33-039
  • Catalogue Number 33-039
  • Brand Family Upstate
  • Trade Name
  • HTRF
  • Upstate
  • PI 3-Kinase Class II HTRF Assay
  • Components
  • Reaction Buffer (4X)
  • DM A Detection Reagent
  • DM C Detection Reagent
  • DM D Detection Reagent
  • Phosphatidylinositol (1mM)
  • Phosphatidylserine (1mM)
  • Stop Reagent C
  • Detection method HTRF
  • Quality Level MQ300
  • Application Five-plate kit (1920 wells) of reagents for performing PI 3-Kinase (Class II) 4-step HTRF assays. Suitable for the following Kinases: PI3KC2α, PI3KC2β & PI3KC2γ.
  • Key Applications
  • Kinase Assay
  • Species Reactivity
  • Human
  • Mouse
  • Analytes Available
  • PI 3-Kinase Class II
  • Entrez Gene Number
  • NM_002645.2
  • NM_002646.3
  • NM_004570.4
  • Entrez Gene Summary Phosphoinositide 3-kinase-C2 proteins generate phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3, 4-bisphosphate (PtdIns(3, 4)P2) that act as second messengers. This protein belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is sensitive to low nanomolar levels of the inhibitor wortmanin. The C2 domain of this protein was shown to bind phospholipids but not Ca2+, which suggests that this enzyme may function in a calcium-independent manner.
  • Gene Symbol
  • PI3KC2α
  • PI3KC2β
  • PI3KC2γ
  • Target Sub-Family PI3K/PI4K
  • Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
  • Storage Conditions 12 months at -70° C
  • Material Size 1920 wells
  • Material Package Kit capacity: 1920 wells

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